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Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer
Dissertation (MSc)--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | en_US |
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University of Pretoria
2020
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| _version_ | 1867613455527182336 |
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| access_status_str | Open Access |
| author2 | Moela, Pontsho |
| author_browse | Moela, Pontsho |
| author_facet | Moela, Pontsho |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/75200 |
| institution | University of Pretoria (South Africa) |
| language | en_US |
| last_indexed | 2026-06-10T12:36:25.392Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/75200 Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer Moela, Pontsho mphoingrid@gmail.com Motadi, Lesetja Mosweu, Mpho UCTD Genetics Dissertation (MSc)--University of Pretoria, 2020. Cancer remains a major health problem. It is ranked the second most common cause of death to cardiovascular diseases worldwide, with about 9.6 million deaths annually. This burden is greatly carried by developing countries, which are accountable for about 65% of cancer death worldwide. Retinoblastoma binding protein 6 (RBBP6) is one of the genes identified as proliferative gene that plays a role in cancer development. It has been shown together with telomerase activity to be highly increased in various cancer cells. E6 protein of human papillomavirus (HPV) and RBBP6 are known to enhance the progression of cancer cells by interacting with p53 and presenting it for ubiquitination by the proteasome, thereby promoting cell proliferation and preventing apoptosis. Studies also show that HPV E6 protein has the ability to increase telomerase activity by activating the expression of human telomerase reverse transcriptase (hTERT) thus, enabling the immortalization of the cells. With RBBP6 and hTERT sharing similar functions, here we seek to investigate possible effect of RBBP6 expression on telomerase activity. Using real time qPCR and TRAPeze RT Telomerase detection kit (Merck, United states), CaSki cells showed that the expression of hTERT and telomerase activity, respectively, were decreased significantly in RBBP6-knockdown cells. However, HeLa cells showed non-significant changes in hTERT expression or telomerase activity in response to RBBP6 silencing. Real-time cell analysis assay revealed a significant cell growth reduction in cells co-silenced for RBBP6 and hTERT, thus substantiating our speculation that RBBP6 and hTERT play an additive role in cell proliferation. Taken all together, RBBP6 expression might be altering telomerase activity in a cell line dependent manner. RBBP6 and TERT co-silencing has an effect on cell proliferation. National Research Foundation (NRF) Genetics Msc Unrestricted 2020-07-14T08:20:19Z 2020-07-14T08:20:19Z 2020 2020 Dissertation Mosweu, M 2020, Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer, Msc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/75200> S2020 http://hdl.handle.net/2263/75200 en_US © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Genetics Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title | Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title_full | Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title_fullStr | Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title_full_unstemmed | Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title_short | Investigating the effects of RBBP6 gene expression on telomerase activity in cervical cancer |
| title_sort | investigating the effects of rbbp6 gene expression on telomerase activity in cervical cancer |
| topic | UCTD Genetics |
| url | http://hdl.handle.net/2263/75200 |