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Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes
Dissertation (MSc)--University of Pretoria, 2020
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613591653318656 |
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| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2020 |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/75249 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:35.295Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/75249 Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes Berger, David Kenneth eugene.kabwe@gmail.com Duong, Tuan A. Kabwe, Eugene N.K. UCTD Disease Fungi Dothideomycete Dissertation (MSc)--University of Pretoria, 2020 In Africa, the grey leaf spot disease in maize is caused by the hemibiotrophic Dothideomycete Cercospora zeina. Dothideomycetes consist of many economically important phytopathogenic fungi. During infection of the host, phytopathogenic fungi secrete effectors that promote infection. In this dissertation, I review how various effectors perform their function. I then review how the “two-speed” genome of many Dothideomycete phytopathogens is important for the gain of virulence and loss of avirulence effector genes in the constant molecular arms race with the plant host. Furthermore, to improve our understanding of the pathogenicity of C. zeina, we aim to identify the effector gene catalogue and analyse its genome architecture. We present a contiguous genome assembly of C. zeina generated by PacBio SMRT sequencing technology. The assembly consists of 17 nuclear genome contigs that make up the 41 Mbp genome and contain three possible full chromosomes. The annotation of the genome has revealed a secretome that contains many proteins predicted to have oxidoreductive and peroxidase activities. Effector prediction revealed a total of 274 effectors which included potential homologues of the ECP2, ECP6 and AVR4 effectors from Cladosporium fulvum. These results imply that C. zeina potentially secretes proteins that prevent recognition by the host and protect against oxidative stress. Additionally, genome architecture analysis of C. zeina has revealed a bipartite structure consisting of 33.2% AT-rich compartments and 66.8% GC-rich compartments. However, effector genes are not concentrated in the AT-rich compartments. This study has paved way for the functional characterization of candidate C. zeina effectors which could ultimately lead to effector-based breeding of maize. This project was supported by grant numbers 118503 and 98977 from the National Research Foundation (NRF) of South Africa Biochemistry MSc Restricted 2020-07-15T12:30:32Z 2020-07-15T12:30:32Z 2020 2020 Dissertation Kabwe, ENK 2020, Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd yymmdd http://hdl.handle.net/2263/51914 S2019 http://hdl.handle.net/2263/75249 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Disease Fungi Dothideomycete Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title | Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title_full | Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title_fullStr | Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title_full_unstemmed | Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title_short | Discovery and genomic architecture of Cercospora zeina (Crous & U. Braun) effector genes |
| title_sort | discovery and genomic architecture of cercospora zeina crous u braun effector genes |
| topic | UCTD Disease Fungi Dothideomycete |
| url | http://hdl.handle.net/2263/75249 |