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Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure
Dissertation (MSc)--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613681015062528 |
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| access_status_str | Open Access |
| author2 | Thantsha, Mapitsi Silvester |
| author_browse | Thantsha, Mapitsi Silvester |
| author_facet | Thantsha, Mapitsi Silvester |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/75513 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:00.333Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/75513 Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure Thantsha, Mapitsi Silvester u04384415@tuks.co.za Mathipa, Moloko Gloria Ncube, Brighton UCTD Microbiology Dissertation (MSc)--University of Pretoria, 2020. Listeria monocytogenes is a foodborne pathogen that causes listeriosis. This study investigates the prevalence of L. monocytogenes in food samples acquired from retail stores and street vendors around Pretoria and evaluates various probiotics as a control measure. The pathogen was isolated by selective enrichment and plating on selective media. All presumptive colonies were confirmed by morphological analysis, 16SrRNA gene BLAST analysis and inlA amplification. Probiotic antagonistic tests against two L. monocytogenes isolates acquired from food samples and three controls was performed. The probiotics used were Bifidobacterium animalis, Lactobacillus acidophilus, Lactobacillus plantarum and Pediococcus acidilacti during spot inoculation tests and well diffusion assays using probiotic cell free supernatants (CFS). The effect probiotics and their cocktails had on avocado and cucumber samples spiked with L. monocytogenes strains was assayed. Of 167 food samples, only two, avocado and cucumber, tested positive for L. monocytogenes. Samples positive for L. monocytogenes were obtained from street vendors exhibiting poor hygiene. B. animalis inhibited growth of four out five L. monocytogenes strains, L. acidophilus three, L. plantarum one while P. acidilacti inhibited none. Inhibition by all probiotic strains, their CFSs and cocktails in both food matrixes was statistically insignificant, except for L. monocytogenes T62 inhibition in avocado by B. animalis. Properties of L. monocytogenes, food medium and storage conditions used in the study could have had an impact on their inhibitory effects. National Research Fund Microbiology and Plant Pathology MSc Unrestricted 2020-07-31T06:29:38Z 2020-07-31T06:29:38Z 2020-09-30 2020-06 Dissertation Ncube, B 2020, Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/75513> S2020 http://hdl.handle.net/2263/75513 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Microbiology Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title | Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title_full | Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title_fullStr | Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title_full_unstemmed | Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title_short | Prevalence of Listeria monocytogenes in food samples from retail shops and street vendor stalls in Pretoria and the evaluation of bacterial probiotics as potential control measure |
| title_sort | prevalence of listeria monocytogenes in food samples from retail shops and street vendor stalls in pretoria and the evaluation of bacterial probiotics as potential control measure |
| topic | UCTD Microbiology |
| url | http://hdl.handle.net/2263/75513 |