Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum
Mini Dissertation (MSc)--University of Pretoria, 2020.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2020
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613729574617088 |
|---|---|
| access_status_str | Open Access |
| author2 | Naidoo, Sanushka |
| author_browse | Naidoo, Sanushka |
| author_facet | Naidoo, Sanushka |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Mini Dissertation (MSc)--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/75549 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:46.593Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/75549 The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum Naidoo, Sanushka smith.robynleigh@gmail.com Coetzee, Martin Petrus Albertus Smith, Robyn Leigh UCTD Genetics Mini Dissertation (MSc)--University of Pretoria, 2020. Pitch canker, caused by Fusarium circinatum (teleomorph Gibberella circinata), is regarded as one of the most severe phytopathogenic diseases affecting commercial Pinus plantations worldwide, and is anticipated to be aggravated by the increase in temperature and humidity associated with climate change. In South Africa, the preferred commercial pine species has been Pinus patula. The fungus causes Fusarium-wilt on seedlings and has drastically decreased the post-planting survival of seedlings. Hybridisation of P. patula with Fusarium-tolerant species such as Pinus tecunumanii, has led to the development of an alternative planting stock. However, the mechanisms underlying P. tecunumanii’s relative resistance remains inconclusive. Previous research has highlighted the putative involvement of terpene synthases in defence against Fusarium spp., as their regulation is significantly altered early in response to this pathogen. RNA-seq reference transcriptomes for P. patula and P. tecunumanii have provided the much-needed genetic resources necessary to interrogate the putative defence related genes undergoing differential expression due to F. circinatum infection. The aim of this study was to determine whether observed differences in resistance between these two hosts, is correlated with induced terpene synthase gene expression profiles, and to resolve the implicated orthologs. The differential expression patterns of genes identified as terpene synthase orthologs was assessed for their potential to contribute to resistance. While the two host species, P. patula and P. tecunumanii, did appear to respond dissimilarly, this is not indicative of terpene synthesis playing a causal role in defence. 1 Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), Genomics Research Institute (GRI), University of Pretoria, Pretoria, South Africa Genetics MSc Unrestricted 2020-08-04T19:16:40Z 2020-08-04T19:16:40Z 2020-09-01 2020 Mini Dissertation * S2019 A2021 http://hdl.handle.net/2263/75549 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Genetics The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title | The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title_full | The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title_fullStr | The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title_full_unstemmed | The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title_short | The partial characterisation of the terpene synthase genes implicated in the defence response by Pinus patula and Pinus tecunumanii to Fusarium circinatum |
| title_sort | partial characterisation of the terpene synthase genes implicated in the defence response by pinus patula and pinus tecunumanii to fusarium circinatum |
| topic | UCTD Genetics |
| url | http://hdl.handle.net/2263/75549 |