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The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis
Thesis (PhD (Medical Immunology))--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613478945030144 |
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| access_status_str | Open Access |
| author2 | Cholo, Moloko C. |
| author_browse | Cholo, Moloko C. |
| author_facet | Cholo, Moloko C. |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Medical Immunology))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/76357 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:45.136Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/76357 The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis Cholo, Moloko C. aymangassim@gmail.com Osman, Ayman Gassim Elamin UCTD Medical Immunology Tuberculosis Mycobacterium tuberculosis Suicide-delivery vector Double-crossover Bacterial growth K+-uptake system Single-crossover Trk and Kdp SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-17: Partnerships for the goals Thesis (PhD (Medical Immunology))--University of Pretoria, 2020. In his thesis, The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis, Mr Osman investigated the roles of the major potassium-uptake transporters utilized by the dangerous bacterial pathogen, Mycobacterium tuberculosis, during bacterial growth. This necessitated generating an Mtb triple-gene-knockout strain in which these potassium transporter-encoding genes were selectively inactivated using a homologous recombination procedure, whereafter the functional roles of these transporters were probed by phenotypic characterisation of the mutant strain extracellularly in planktonic and biofilm cultures and intracellularly in macrophages. He demonstrated that the mutagenesis tools were effective in generating an Mtb triple-gene-knockout mutant strain. Deletion of these transporters affected bacterial growth in the three environments, which manifested as an increase in planktonic growth, attenuation of biofilm formation and a decrease in macrophage intracellular survival. The findings demonstrated the essentiality of these bacterial transporters during various bacterial growth stages, underscoring their potential as novel drug and vaccine targets. em2026 Immunology PhD (Medical Immunology) Unrestricted SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-17: Partnerships for the goals 2020-10-06T08:50:49Z 2020-10-06T08:50:49Z 2020-04-04 2020 Thesis Osman, AGE 2020, the roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis, PhD thesis, University of Pretoria, Pretoria, viewed 200201 http://hdl.handle.net/2263/76357 A2020 http://hdl.handle.net/2263/76357 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Medical Immunology Tuberculosis Mycobacterium tuberculosis Suicide-delivery vector Double-crossover Bacterial growth K+-uptake system Single-crossover Trk and Kdp SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-17: Partnerships for the goals The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title | The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title_full | The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title_fullStr | The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title_full_unstemmed | The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title_short | The roles of the potassium-uptake systems, Trk and Kdp, in the extracellular and intracellular growth of Mycobacterium tuberculosis |
| title_sort | roles of the potassium uptake systems trk and kdp in the extracellular and intracellular growth of mycobacterium tuberculosis |
| topic | UCTD Medical Immunology Tuberculosis Mycobacterium tuberculosis Suicide-delivery vector Double-crossover Bacterial growth K+-uptake system Single-crossover Trk and Kdp SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-17: Partnerships for the goals |
| url | http://hdl.handle.net/2263/76357 |