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Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique

Dissertation (MSc)--University of Pretoria, 2019.

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Other Authors: Neves, Luís C.B.G.
Format: Thesis
Language:English
Published: University of Pretoria 2020
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access_status_str Open Access
author2 Neves, Luís C.B.G.
author_browse Neves, Luís C.B.G.
author_facet Neves, Luís C.B.G.
collection Thesis
dc_rights_str_mv © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc)--University of Pretoria, 2019.
format Thesis
id oai:repository.up.ac.za:2263/76721
institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:40:32.922Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2020
publishDateRange 2020
publishDateSort 2020
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/76721 Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique Neves, Luís C.B.G. u16401809@tuks.co.za Sibeko-Matjila, K.P. (Kgomotso Penelope) Mkhize, Smangele R. UCTD Dissertation (MSc)--University of Pretoria, 2019. Theileria parva is the most significant tick-borne haemoparasite associated with buffalo and it causes Corridor disease in Southern Africa. African buffalo (Syncerus caffer) is known for being the host of many pathogens causing diseases of veterinary importance, resulting in severe economic loss. The objective of this study was to detect haemoparasites species in blood samples obtained from a buffalo herd in the Zambezi delta in Mozambique, using DNA-based diagnostic assays. Several tick-borne blood-parasites namely Theileria, Babesia, Anaplasma and Ehrlichia species were detected, either as single or mixed infections, with the use of the reverse line blot hybridization assay. Furthermore, Theileria parva infections were confirmed by the quantitative real-time PCR (qPCR). This is the first report of tick-borne haemoparasites in the area of Zambezi delta in Mozambique. The results of this study indicate the occurrence of the following tick-borne haemoparasites in the Zambezi delta buffalo population. Theileria parva (95%) was the most frequently found species followed by Theileria sp. (sable) (85%), A. marginale (63%), T. velifera, (45%), T. mutans (43%), A. centrale (10%), T. sp. (buffalo) (3%) finally Anaplasma sp. (Omatjienne) (3%). These results strongly indicate a high challenge of the buffalo population in this area by tick-borne haemoparasites, especially T. parva. The qPCR was the most sensitive technique for detection of T. parva, detecting this pathogen in 100% of tested samples compared to 95% by RLB. The high occurrence of T. parva and A. marginale infections in this herd, the causative agents of the fatal bovine theileriosis and bovine anaplasmosis may pose a risk to the cattle population in the surrounding areas. Veterinary Tropical Diseases MSc Unrestricted 2020-11-04T15:09:42Z 2020-11-04T15:09:42Z 2020/04/22 2019 Dissertation Mkhize, SR 2019, Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76721> A2020 http://hdl.handle.net/2263/76721 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle UCTD
Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title_full Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title_fullStr Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title_full_unstemmed Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title_short Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique
title_sort detection of tick borne haemoparasites in an isolated buffalo syncerus caffer population from the zambezi delta in mozambique
topic UCTD
url http://hdl.handle.net/2263/76721