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Dissertation (MSc)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613715030867969 |
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| access_status_str | Open Access |
| author2 | Neves, Luís C.B.G. |
| author_browse | Neves, Luís C.B.G. |
| author_facet | Neves, Luís C.B.G. |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/76721 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:32.922Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/76721 Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique Neves, Luís C.B.G. u16401809@tuks.co.za Sibeko-Matjila, K.P. (Kgomotso Penelope) Mkhize, Smangele R. UCTD Dissertation (MSc)--University of Pretoria, 2019. Theileria parva is the most significant tick-borne haemoparasite associated with buffalo and it causes Corridor disease in Southern Africa. African buffalo (Syncerus caffer) is known for being the host of many pathogens causing diseases of veterinary importance, resulting in severe economic loss. The objective of this study was to detect haemoparasites species in blood samples obtained from a buffalo herd in the Zambezi delta in Mozambique, using DNA-based diagnostic assays. Several tick-borne blood-parasites namely Theileria, Babesia, Anaplasma and Ehrlichia species were detected, either as single or mixed infections, with the use of the reverse line blot hybridization assay. Furthermore, Theileria parva infections were confirmed by the quantitative real-time PCR (qPCR). This is the first report of tick-borne haemoparasites in the area of Zambezi delta in Mozambique. The results of this study indicate the occurrence of the following tick-borne haemoparasites in the Zambezi delta buffalo population. Theileria parva (95%) was the most frequently found species followed by Theileria sp. (sable) (85%), A. marginale (63%), T. velifera, (45%), T. mutans (43%), A. centrale (10%), T. sp. (buffalo) (3%) finally Anaplasma sp. (Omatjienne) (3%). These results strongly indicate a high challenge of the buffalo population in this area by tick-borne haemoparasites, especially T. parva. The qPCR was the most sensitive technique for detection of T. parva, detecting this pathogen in 100% of tested samples compared to 95% by RLB. The high occurrence of T. parva and A. marginale infections in this herd, the causative agents of the fatal bovine theileriosis and bovine anaplasmosis may pose a risk to the cattle population in the surrounding areas. Veterinary Tropical Diseases MSc Unrestricted 2020-11-04T15:09:42Z 2020-11-04T15:09:42Z 2020/04/22 2019 Dissertation Mkhize, SR 2019, Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76721> A2020 http://hdl.handle.net/2263/76721 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title | Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title_full | Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title_fullStr | Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title_full_unstemmed | Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title_short | Detection of tick-borne haemoparasites in an isolated buffalo (Syncerus caffer) population from the Zambezi delta in Mozambique |
| title_sort | detection of tick borne haemoparasites in an isolated buffalo syncerus caffer population from the zambezi delta in mozambique |
| topic | UCTD |
| url | http://hdl.handle.net/2263/76721 |