Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples
Dissertation (MSc)--University of Pretoria, 2019.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2020
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613481704882176 |
|---|---|
| access_status_str | Open Access |
| author2 | Qekwana, Daniel Nenene |
| author_browse | Qekwana, Daniel Nenene |
| author_facet | Qekwana, Daniel Nenene |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/76760 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:50.456Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/76760 Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples Qekwana, Daniel Nenene u13235291@tuks.co.za Petzer, Inge-Marie Phophi, Lufuno UCTD Antimicrobial resistance Milk Staphylococcus Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Dissertation (MSc)--University of Pretoria, 2019. Increased prevalence of antimicrobial resistance, treatment failure, and financial losses have been reported in dairy cattle with coagulase-negative Staphylococcus (CoNS) clinical mastitis. However, studies on CoNS are limited in South Africa. Therefore, the objectives of this study were to investigate the antimicrobial resistance patterns and biofilm formation in CoNS isolated from cow milk samples submitted to the Onderstepoort Milk Laboratory. A total of 142 confirmed CoNS isolates were used for this study. Isolates were subjected to the tissue culture plate method for biofilm formation testing and antimicrobial susceptibility testing against a panel of 11 antimicrobials using the disk diffusion method. Biofilm formation was identified in 18% of CoNS tested. Staphylococcus chromogenes (11%) had the highest proportion of biofilm formation followed by S. haemolyticus 4.0% and S. epidermidis, S. hominis, S. xylosus, and S. simulans with 1% respectively. Ninety percent (90%) of CoNS isolates were resistant to at least one antimicrobial (AMR) and 51% were multidrug-resistant (MDR). Resistance among CoNS was the highest to ampicillin (90%) and penicillin (89%), with few isolates resistant to cefoxitin and vancomycin, 9% respectively. The most common resistance patterns among the CoNS was penicillin-ampicillin (16%) and penicillin-ampicillin-erythromycin (10%). Forty-two percent (42%) of biofilm positive CoNS were MDR. At the species level, MDR was common among S. epidermis (65%), S. chromogenes (52%) and S. haemolyticus (44%). In conclusion, biofilm formation was uncommon among the MDR-CoNS isolates in this study suggesting that biofilm formation is not a major contributing factor to antimicrobial resistance in this study. In addition, most CoNS isolates in this study were _-lactams resistant. This is concerning as penicillins are used commonly by dairy farmers in treatment of mastitis in South Africa. Nonetheless, the role of antimicrobial use practice in the development of resistance in subclinical mastitis in the dairy industry should be investigated. es2026 Paraclinical Sciences MSc Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2020-11-04T15:09:48Z 2020-11-04T15:09:48Z 2020/04/22 2019 Dissertation Phophi, L 2019, Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76760> A2020 http://hdl.handle.net/2263/76760 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Antimicrobial resistance Milk Staphylococcus Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title | Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title_full | Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title_fullStr | Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title_full_unstemmed | Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title_short | Antimicrobial resistance patterns and biofilm formation of coagulase negative Staphylococcus species isolated from cow milk samples |
| title_sort | antimicrobial resistance patterns and biofilm formation of coagulase negative staphylococcus species isolated from cow milk samples |
| topic | UCTD Antimicrobial resistance Milk Staphylococcus Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/76760 |