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Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti
Dissertation (MSc)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613681017159680 |
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| access_status_str | Open Access |
| author2 | Neves, Luís C.B.G. |
| author_browse | Neves, Luís C.B.G. |
| author_facet | Neves, Luís C.B.G. |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/76793 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:00.333Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/76793 Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti Neves, Luís C.B.G. u14023190@tuks.co.za Snyman, Louwtjie P. Smit, Andeliza UCTD Veterinary science theses SDG-01 SDG-01: No poverty Dissertation (MSc)--University of Pretoria, 2019. Tabanidae (Diptera) is a diverse haematophagous fly family, known to transmit over 35 livestock pathogens both mechanically and biologically. Kruger National Park (KNP) (in Limpopo and Mpumalanga provinces), South Africa, has a high diversity of tabanids, representing 26 of the total indigenous species (n=213) present in South Africa. Little modern taxonomical work has been done on tabanids within South Africa despite their medical, veterinary and environmental importance. This study aimed to determine a cost-effective DNA extraction method as well as comparing the traditional alpha-taxonomic approach to species delimitation with molecular methods using two gene regions, mitochondrial cytochrome oxidase I (COI) and nuclear alanyl-tRNA-synthetase (AATS) of tabanids collected in KNP. Furthermore, the study aimed to elucidate the role of tabanids in the transmission of Besnoitia besnoiti as this pathogen has found to ciculate in impala (Aepyceros melampus) and blue wildebeest (Connochaetes taurinus). A comparative study on DNA extraction methods were conducted, of which the most effective method was selected for DNA extractions. Tabanids were captured in three locations within KNP. The flies were morphologically identified then homogenized. DNA was pooled for the B. besnoiti screening, followed by sequencing and phylogenetic analysis. In total, 856 flies were captured belonging to 16 species under five genera. The COI barcode indicated that some species are genetically uniform while others formed co-occurring haplotypes. This study found that COI alone was not adequate in distinguishing between all the species of the South African Tabanidae. It is apparent that the classification of Tabanidae should be placed under scrutiny. A larger sample size, especially with regards to the Tabanus genus, or the use of several markers will aid in clarifying their relationships. No B. besnoiti positives were detected in the screened tabanids. Furthermore, in-depth research should also be conducted in other regions of South Africa; not only on tabanid ecology and composition but their role as pathogen vectors. Veterinary Tropical Diseases MSc Unrestricted 2020-11-04T15:09:53Z 2020-11-04T15:09:53Z 20/04/22 2019 Dissertation Smit, A 2019, Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76793> A2020 http://hdl.handle.net/2263/76793 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Veterinary science theses SDG-01 SDG-01: No poverty Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title | Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title_full | Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title_fullStr | Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title_full_unstemmed | Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title_short | Abundance, composition and barcoding of Tabanidae in Kruger National Park and screening for Besnoitia besnoiti |
| title_sort | abundance composition and barcoding of tabanidae in kruger national park and screening for besnoitia besnoiti |
| topic | UCTD Veterinary science theses SDG-01 SDG-01: No poverty |
| url | http://hdl.handle.net/2263/76793 |