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Effects of storage time and temperature on thromboelastographic analysis in dogs and horses
Dissertation (MSc)--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613592727060480 |
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| access_status_str | Open Access |
| author2 | Hooijberg, Emma Henriette |
| author_browse | Hooijberg, Emma Henriette |
| author_facet | Hooijberg, Emma Henriette |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/76882 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:35.948Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/76882 Effects of storage time and temperature on thromboelastographic analysis in dogs and horses Hooijberg, Emma Henriette u12048722@tuks.co.za Goddard, Amelia Lemon, Ashleigh Valarie UCTD Dissertation (MSc)--University of Pretoria, 2019. Thromboelastography (TEG) is an assay that (to date) provides the most extensive analysis of haemostasis. Accessibility of TEG to general practitioners is limited by short sample storage times (30 minutes) and storage temperature (20_23_C). This limited accessibility is evident in human and veterinary medicine, but especially affects veterinary general practitioners in the field. This study evaluated the stability of canine and equine citrated blood samples when stored for extended periods of time, both at room temperature (RT) (20_23_C) and refrigeration temperature (FT) (2_7.5_C). Citrated whole blood samples from healthy dogs and horses (n=10 for each) were stored for 30 minutes at RT before TEG analysis. Baseline values for TEG variables R, K, _, MA, LY30 and LY60 were compared to those from samples stored for 2 hours, 8 hours, and 22.5 hours, both at RT and FT. Results were compared using ANOVA (p < 0.05) and reference intervals. In dogs, statistically significant differences included shorter R, longer K, decreased MA, and increased LY60 at various time points and storage temperatures from 2 hours. R, _, _ and MA values were stable at 8 hours FT. No clinically relevant differences in R, _, or MA values were found at 2 hours RT or FT. In horses, statistically significant difference included shorter R and K, and decreased _, LY30 and LY60 at various time points and storage temperatures from 2 hours. R, _, _ and MA values were stable at 2 hours and 8 hours FT. No clinically relevant differences in R, _, or MA values were found at 2 hours RT. This study suggests that canine samples can be stored for up to 2 hours at RT or FT and equine samples can be stored for up to 2 hours at RT without affecting TEG results. Companion Animal Clinical Studies MSc Unrestricted 2020-11-04T15:10:15Z 2020-11-04T15:10:15Z 20/04/22 2019 Dissertation Lemon, AV 2019, Effects of storage time and temperature on thromboelastographic analysis in dogs and horses, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/76882> A2020 http://hdl.handle.net/2263/76882 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title | Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title_full | Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title_fullStr | Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title_full_unstemmed | Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title_short | Effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| title_sort | effects of storage time and temperature on thromboelastographic analysis in dogs and horses |
| topic | UCTD |
| url | http://hdl.handle.net/2263/76882 |