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Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands
Dissertation (MSc (Genetics))--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613588154220544 |
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| access_status_str | Open Access |
| author2 | Makhalanyane, Thulani Peter |
| author_browse | Makhalanyane, Thulani Peter |
| author_facet | Makhalanyane, Thulani Peter |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/77020 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:30.789Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/77020 Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands Makhalanyane, Thulani Peter manchamabaso@gmail.com Mabaso, Mancha Microbial Ecology Genetics Biogeochemistry Marine Microbiome UCTD Dissertation (MSc (Genetics))--University of Pretoria, 2020. Recent studies have shown that a range of biological and oceanographic features shape the composition, structure and function of Southern Ocean (SO) microbiomes. Within the vicinity of islands, the interactions between landmasses, the surrounding waters and biology result in complex tropic interactions. These interactions are expected to affect nutrient cycling and ecosystem services the bulk of which is driven by microorganisms. However, little is known regarding the microbial dynamics of near- shore regions of SO islands such as the Prince Edward Islands (PEIs). This knowledge deficit is particularly true for particle attached (PA) and free living (FL) bacterioplankton and fungal microbiota. Here, we provide insights regarding microbial community structure and composition of PEI microbiomes. We assess the effects of physicochemical factors on PEI microbial communities. Samples were collected from three depths, namely, the Fluorescence maximum (Fmax), the oxygen minimum zone (OMZ) and the bathypelagic zone (Deep) at 10 sites within the vicinity of the PEIs. Phylogenetic analysis of 16S and ITS rRNA gene regions and statistical analysis revealed a shift in bacterioplankton and fungal community structure and composition, respectively. This shift coincided with differences in depth and water mass specificity. The results also show that PA and FL PEI microbial communities were strongly influenced by oceanographic features and physicochemical variables. Extracellular enzymatic activities specific for carbon, nitrogen and phosphorus acquisition did not reveal any specific trends between community composition and potential functionality but exhibited a close linkage with substrate availability and depth. These resultssuggest that potential extracellular enzymatic functionality of the microbiome is driven by nutrient dynamics in this environment. This study provides valuable insights regarding the influence of biotic and abiotic drivers in the dynamics of near-shore microbial communities in the SO. University of Pretoria NRF (SANAP) Genetics MSc (Genetics) Unrestricted 2020-11-16T08:29:35Z 2020-11-16T08:29:35Z 2021-04 2020 Dissertation Mabaso, M 2020, Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands, MSc dissertation, University of Pretoria, viewed yymmdd http://hdl.handle.net/2263/31804 http://hdl.handle.net/2263/77020 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Microbial Ecology Genetics Biogeochemistry Marine Microbiome UCTD Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title | Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title_full | Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title_fullStr | Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title_full_unstemmed | Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title_short | Elucidating the effects of physicochemical variables on the structure, composition and functionality of microbiomes in the Prince Edward Islands |
| title_sort | elucidating the effects of physicochemical variables on the structure composition and functionality of microbiomes in the prince edward islands |
| topic | Microbial Ecology Genetics Biogeochemistry Marine Microbiome UCTD |
| url | http://hdl.handle.net/2263/77020 |