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Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2019.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2020
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| _version_ | 1867613489268260864 |
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| access_status_str | Open Access |
| author2 | Sibeko-Matjila, K.P. (Kgomotso Penelope) |
| author_browse | Sibeko-Matjila, K.P. (Kgomotso Penelope) |
| author_facet | Sibeko-Matjila, K.P. (Kgomotso Penelope) |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/77442 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:57.501Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/77442 Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites Sibeko-Matjila, K.P. (Kgomotso Penelope) borchers.lauren17@gmail.com Tjale, Mabotse A. Borchers, Lauren-Leigh UCTD Veterinary science theses SDG-01 SDG-01: No poverty Dissertation (MSc (Veterinary Science))--University of Pretoria, 2019. Theileriosis is a lymphoproliferative tick-borne disease of cattle and other wild ruminants, caused by infection with a protozoan, Theileria parva. The disease is prevalent in cattle throughout Central, East and southern Africa, where it threatens 50% of the cattle population. There are various control and treatments methods used against theileriosis; however, they all have limitations. The available live immunisation method, the Muguga cocktail, does not confer protection against all field strains, particularly buffalo-derived T. parva. Attempts to develop a subunit vaccine have been promising but these have shown limited efficacy due to antigenic and genetic diversity of T. parva strains in the field. Thus, there is a need to search for additional vaccine candidates. A related study has identified potential vaccine candidates using a genome-wide in silico approach. Consequently, the aim of this study was to genotype one of the identified antigens. TP04_0028 was selected for genotyping among candidate genes with high expression levels in the schizont stage of both cattle- and buffalo-derived T. parva isolates. Specific primers were designed and optimised for PCR amplification and sequencing. The comprehensive analysis of sequences from 17 cattle- and 17 buffalo-derived T. parva, from East and southern Africa, showed conservation in 12 (60%) of the 20 TP04_0028 predicted epitopes, in both parasite types, irrespective of geographical origin. Eighteen of the 20 predicted epitopes are conserved amongst different BoLA alleles and an area of 7 overlapping epitopes could be the starting point for initial experimental evaluation of the immunogenic properties of TP04_0028. Once the immunogenicity of these epitopes have been tested and the extent to provide protection from cattle- and buffalo-derived infections have been verified, they may be considered for vaccine development. Veterinary Tropical Diseases MSc (Veterinary Science) Unrestricted 2020-12-21T09:53:46Z 2020-12-21T09:53:46Z 2020/09/30 2020 Dissertation Borchers, L 2020, Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites, MSc (Veterinary Science) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/77442> S2020 http://hdl.handle.net/2263/77442 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Veterinary science theses SDG-01 SDG-01: No poverty Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title | Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title_full | Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title_fullStr | Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title_full_unstemmed | Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title_short | Genotyping a novel Theileria parva candidate vaccine antigen in cattle- and buffalo-derived parasites |
| title_sort | genotyping a novel theileria parva candidate vaccine antigen in cattle and buffalo derived parasites |
| topic | UCTD Veterinary science theses SDG-01 SDG-01: No poverty |
| url | http://hdl.handle.net/2263/77442 |