Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules
Dissertation (MSc)--University of Pretoria, 2019.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2020
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613482565763072 |
|---|---|
| access_status_str | Open Access |
| author2 | Makgopa, M.E. (Matome Eugene) |
| author_browse | Makgopa, M.E. (Matome Eugene) |
| author_facet | Makgopa, M.E. (Matome Eugene) |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/77827 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:51.203Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/77827 Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules Makgopa, M.E. (Matome Eugene) u11160625@tuks.co.za Hlokwe, Mogale Clinton UCTD Dissertation (MSc)--University of Pretoria, 2019. Pre-mature nodule senescence is a naturally occurring process that often has a negative impact on plant growth and development. Nodule senescence is regulated by a class of enzymes known as cysteine proteases. In this study, transgenic soybean (Glycine max) were investigated in order to determine whether nodule senescence can be prolonged by reducing the activity of cysteine proteases. Transgenic lines over-expressing a cysteine protease inhibitor (oryzacystatin-I) in root nodules were characterized under different growth conditions. Differential transgene expression occurred in transgenic lines. The presence of OCI was detected by determining cysteine protease activity using a fluoremetric assay which utilizes a fluorogenic cysteine protease substrate. Under well-watered conditions, OCI did lower cysteine protease activity in transgenic plants and also improved the growth rate of trifoliate leaves. The most potent effects of OCI were observed when plants were less than 9 weeks old. This was maybe due to a spike of OCI expression in nodules. Although cysteine protease activity was also lowered under drought conditions, detectable effects of OCI on plant biology were difficult to characterize in drought-treated transgenic lines. Overall, the results of this study demonstrated that organ-specific over-expression of a cysteine protease inhibitor can alter certain plant functionalities that are related to the activity of those cysteine proteases. Future work will focus on determining OCI sensitive proteases and the respectable pathways which they are involved in. Plant Production and Soil Science MSc Unrestricted 2020-12-29T11:50:49Z 2020-12-29T11:50:49Z 2020/04/24 2019 Dissertation Hlokwe, MC 2019, Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/77827> A2020 http://hdl.handle.net/2263/77827 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title | Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title_full | Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title_fullStr | Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title_full_unstemmed | Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title_short | Molecular and physiological characterization of transgenic soybean (Glycine max) over-expressing oryzacystatin-I (OCI) in their root nodules |
| title_sort | molecular and physiological characterization of transgenic soybean glycine max over expressing oryzacystatin i oci in their root nodules |
| topic | UCTD |
| url | http://hdl.handle.net/2263/77827 |