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Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis
Thesis (PhD (Medical Microbiology))--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2021
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| _version_ | 1867613517413089280 |
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| access_status_str | Open Access |
| author2 | Fourie, Bernard P. |
| author_browse | Fourie, Bernard P. |
| author_facet | Fourie, Bernard P. |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Medical Microbiology))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/78299 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:24.530Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/78299 Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis Fourie, Bernard P. sbongakee@yahoo.com Rossouw, Theresa M. Shey, Bong-Akee UCTD SDG-03: Good health and well-being Thesis (PhD (Medical Microbiology))--University of Pretoria, 2020. This study demonstrates the ability of a selected novel IgG1 mouse monoclonal antibody, JG7 III D3 I F9 (JG7), to enhance the phagocytic elimination (opsonophagocytosis) of susceptible M. tuberculosis strains at both their mid-logarithmic and stationary growth phases, by human U-937 macrophage and HL-60 granulocyte cell lines which are involved in intracellular killing of bacteria, and to assess the cytokine response to this process. The assays showed JG7-enhanced phagocytosis to all M. tuberculosis strains used, though at different levels. There was a relative increase in OPKA with increase in mAb concentration, at each bacterial dilution assessed (1:100 and 1:1000), and this increase was demonstrated mostly with the cMtb strain by the U-937 macrophage cell line. For the multiplex suspension array assays, the JG7 concentration of 25 µg/mL was selected, since it was used in both binding and OP assays. Results showed that the mAb induced a possible pro-inflammatory effect, driven by IL-8, in neutrophils and a significant anti-inflammatory response in MNL cells, as demonstrated by the significant decrease in pro-inflammatory IL-12p70 and increase in anti-inflammatory IL-10. em2026 Medical Microbiology PhD (Medical Microbiology) Unrestricted SDG-03: Good health and well-being 2021-02-08T10:58:49Z 2021-02-08T10:58:49Z 2021 2020 Thesis Shey, BA 2020, Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis, PhD thesis, University of Pretoria, Pretoria http://hdl.handle.net/2263/78299 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD SDG-03: Good health and well-being Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title | Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title_full | Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title_fullStr | Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title_full_unstemmed | Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title_short | Assessment of macrophage and granulocyte cell-enhanced phagocytosis of Mycobacterium tuberculosis |
| title_sort | assessment of macrophage and granulocyte cell enhanced phagocytosis of mycobacterium tuberculosis |
| topic | UCTD SDG-03: Good health and well-being |
| url | http://hdl.handle.net/2263/78299 |