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Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis
Dissertation (MSc)--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2021
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| _version_ | 1867613516139069440 |
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| access_status_str | Open Access |
| author2 | Mabeta, Peaceful |
| author_browse | Mabeta, Peaceful |
| author_facet | Mabeta, Peaceful |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/78412 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:23.306Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/78412 Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis Mabeta, Peaceful u19156759@tuks.co.za Skepu, Amanda Bida, Meshack Matutule, Lebogang Johanna Gold nanoparticles Tumour angiogenesis Melanoma Angiogenesis proteins (VEGF-A and PIGF) UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 Dissertation (MSc)--University of Pretoria, 2020. Melanoma is a skin cancer that relies on angiogenesis for growth and progression. Angiogenesis is the growth of new vessels from existing vessels and follows a number of steps that include endothelial cell growth, migration and tubulogenesis. Current anti-angiogenic drugs are not effective in the treatment of melanomas due to serious side effects such as hypertension and the development of resistance. On the other hand, gold nanoparticles (AuNPs) have been reported to be biocompatible in preclinical models. Furthermore, AuNPs were shown to be cytotoxic to prostate cancer cells. In human umbilical vein endothelial cells, AuNPs inhibited the angiogenic protein, vascular endothelial growth factor-A (VEGF-A). Therefore, the study aimed to investigate the possible cytotoxic effects of AuNPs (1.2–3.2 nM) on melanoma cells and angiogenesis parameters (endothelial cell growth and migration) as well as on the levels of angiogenesis promoting proteins, VEGF-A and placental growth factor (PIGF). Melanoma (B16-F10) cells and tumour-derived endothelial (sEnd.2) cells were maintained in an incubator in a humidified atmosphere containing 5% CO2 at a temperature of 37°C. To investigate whether AuNPs were cytotoxic to melanoma cells, the effect of the particles on B16-F10 cell survival was measured using the crystal violet assay. To determine the effects of AuNPs on angiogenesis parameters, endothelial cell (EC) growth and migration were investigated using crystal violet assay and the scratch assay respectively. Also, EC morphology was studied using polarisation-optical interference contrast light microscopy. The enzyme-linked immunosorbent assay (ELISA) was used to determine the effects of AuNPs on the levels of VEGF-A and PIGF. The results showed that AuNPs decreased the viability of melanoma and endothelial cells. The scratch assay showed that more ECs migrated in cultured treated with AuNPs (P < 0.05). The concentration of VEGF-A and PIGF was reduced significantly following treatment with AuNPs, meaning that the particles exhibited anti-angiogenic properties. This outcome provides a basis for further testing of AuNPs as a potential treatment for melanoma. National Research Foundation Mintek em2026 Physiology MSc Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2021-02-10T15:39:04Z 2021-02-10T15:39:04Z 2021-04 2020-11 Dissertation Matutule, LJ 2020, Effect of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd http://hdl.handle.net/2263/78412 A2021 http://hdl.handle.net/2263/78412 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Gold nanoparticles Tumour angiogenesis Melanoma Angiogenesis proteins (VEGF-A and PIGF) UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title | Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title_full | Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title_fullStr | Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title_full_unstemmed | Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title_short | Effects of gold nanoparticles on vascular endothelial growth factor-A-induced melanoma cell growth and angiogenesis |
| title_sort | effects of gold nanoparticles on vascular endothelial growth factor a induced melanoma cell growth and angiogenesis |
| topic | Gold nanoparticles Tumour angiogenesis Melanoma Angiogenesis proteins (VEGF-A and PIGF) UCTD Health sciences theses SDG-03 Health sciences theses SDG-17 |
| url | http://hdl.handle.net/2263/78412 |