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Fusarium species diversity from maize and teff in the northern Free State
Dissertation (MSc (Genetics))--University of Pretoria 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2021
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| _version_ | 1867613542303137792 |
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| access_status_str | Open Access |
| author2 | Van der Merwe, Nicolaas Albertus (Albie) |
| author_browse | Van der Merwe, Nicolaas Albertus (Albie) |
| author_facet | Van der Merwe, Nicolaas Albertus (Albie) |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/78951 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:48.227Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/78951 Fusarium species diversity from maize and teff in the northern Free State Van der Merwe, Nicolaas Albertus (Albie) pozisamajaja@gmail.com Yilmaz, Neriman Majaja, Pozisa Sinayo UCTD Species diversity of Fusarium species on maize Dissertation (MSc (Genetics))--University of Pretoria 2021. Fusarium is a ubiquitous mycotoxigenic genus of filamentous fungi. Members of this genus cause devastating plant diseases on various cereal grains such as maize, rice, wheat, and sorghum. Maize (Zea mays) is the most widely produced cereal grain globally, with over 1.2 billion tons produced in 2019 (FAO, 2020). In Southern Africa, maize is a staple food that is consumed by more than 70% of the population, however Fusarium species cause many diseases on maize and subsequently reduces grain yield and quality. The additional health risks posed by mycotoxins greatly impacts both the health system as well as the food industry. Teff (Eragrostis tef) is a cereal grain that is primarily used as animal feed and a staple food consumed by humans in East Africa. There is limited knowledge on Fusarium species diversity, and the impact of such species, on teff. This study aimed to investigate the species diversity of Fusarium from asymptomatic transgenic maize intended for human consumption, as well as teff. A total of 102 Fusarium-like isolates were identified morphologically. Diagnosis was established by using polymerase chain reaction of fungal DNA, followed by sequencing portions of translation elongation factor 1 alpha (TEF-1α), RNA polymerase second largest subunit (RPB2), calmodulin (CAL) and beta-tubulin (β-TUB). The multi-locus maximum likelihood phylogenies revealed 80 Fusarium isolates. The isolates consisted of five species complexes, namely the Fusarium sambucinum -, incarnatum-equiseti -, fujikuroi -, oxysporum -, and chlamydosporum species complexes. Some recovered species are known to be pathogenic and mycotoxigenic, such as Fusarium verticillioides, F. temperatum, F. boothii, F. subglutinans, and F. nygamai. This study has resulted in the first report of many species on maize and teff in South Africa and widened our understanding of this genus and its distribution as well as its association with maize and teff. The co-occurrence of various Fusarium species from each plant was observed, raising the possibility of various cocktails of mycotoxins present on these plants. Further phylogenetic analysis resulted in the description of one novel Fusarium species (Fusarium iowaense prov. nom.) within the main African clade of the FFSC. TPCP and CPHB Genetics MSc (Genetics) Unrestricted 2021-03-05T06:21:09Z 2021-03-05T06:21:09Z 2021 2021 Dissertation * A2021 http://hdl.handle.net/2263/78951 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Species diversity of Fusarium species on maize Fusarium species diversity from maize and teff in the northern Free State |
| title | Fusarium species diversity from maize and teff in the northern Free State |
| title_full | Fusarium species diversity from maize and teff in the northern Free State |
| title_fullStr | Fusarium species diversity from maize and teff in the northern Free State |
| title_full_unstemmed | Fusarium species diversity from maize and teff in the northern Free State |
| title_short | Fusarium species diversity from maize and teff in the northern Free State |
| title_sort | fusarium species diversity from maize and teff in the northern free state |
| topic | UCTD Species diversity of Fusarium species on maize |
| url | http://hdl.handle.net/2263/78951 |