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Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase
Dissertation (MSc)--University of Pretoria, 2015.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2021
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| _version_ | 1867613507574300672 |
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| access_status_str | Open Access |
| author2 | Lall, Namrita |
| author_browse | Lall, Namrita |
| author_facet | Lall, Namrita |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/79237 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:15.129Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/79237 Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase Lall, Namrita nomasomi.gasa2gmail.com Gasa, Nomasomi UCTD Antibiofilm activity Biofilm inhibition Mycobacterium spp. Mycobacterial infections Plant extracts Phytochemicals Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 Dissertation (MSc)--University of Pretoria, 2015. Tuberculosis (TB) is an infectious disease, mostly affecting lungs or abdominal area. The causative agent of TB is a bacterium called Mycobacterium tuberculosis; it is the second leading cause of death in the world. Plants have been considered as a possible additive to current treatment, due to resistance of M. tuberculosis to commercially available drugs. Twenty South African medicinal plants ethanol extracts, tradititonally used to treat TB related symptoms were evaluated for their antituberculosis activity. Twenty South African medicinal plant extracts were evaluated for in vitro antimycobacterial activities using the Microtiter Alamar Blue Assay and cytotoxicity with XTT (2, 3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay. Mycobacterium tuberculosis H37Rv strain was used as the test organism. Cytotoxicity test was done on human macrophages (U937 cell line). Of all the 20 extracts 7 showed activities against Mycobacterium with minimum inhibitory concentrations ranging from 125-31.25 μg/ml. Ficus sur had a selectivity index of 3 followed by Salvia africana-lutea and Sphedamnocarpus pruriens with selectivity indexes of 2. The plant extracts were further tested for their activity against Glutathione disulfide reductase (Human analogue) and Mycothiol disulfide reductase (Mycobacterium analogue). The inhibitory activity of the plant extracts was determined using a DTNB-coupled Glutathione/Mycothiol disulfide reductase assay. Typha minima showed a potential inhibitory activity against Mycothione reductase (Mtr) with an effective concentration at which 50% activity is inhibited (EC50) of 47.89±47.5μg/ml and had less inhibitory activity against Glutathione reductase (Gtr) with an EC50 of 813.5±3.21μg/ml. The plant extracts were screened to evaluate whether they had antimicrobial and antibiofilm formation activity, utilizing Mycobacterium smegmatis as the test organism, which is a genetic homologue to Mycobacterium tuberculosis. No significant antimicrobial activity was observed from the plant extracts. Based on a visual analysis the plant extracts; Sphedamnocarpus pruriens (N), Salvia africana-lutea (L), Withania somnifera (R) showed considerable antibiofilm activity as compared to Ciproflaxicin (positive control) displayed a better inhibitory activity and was validated by determining quantitavely using crystal violet absorption. The effective concentration (EC50) was determined and Leonotis leonurus L. (K), Salvia africana-lutea (L), and Sphedamnocarpus pruriens (N) showed potential biofilm formation inhibition at concentration 45.55±0.2475μg/ml, 100±56.83μg/ml and 61.39±60.59μg/ml respectively when compared to Ciproflaxicin, which inhibited at a concentration of 1.9802μg/ml. bs2026 Plant Science MSc Unrestricted SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure SDG-15: Life on land 2021-04-06T07:22:21Z 2021-04-06T07:22:21Z 2015/07/09 2015 Dissertation Gasa, N 2015, Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/79237> S2015 http://hdl.handle.net/2263/79237 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Antibiofilm activity Biofilm inhibition Mycobacterium spp. Mycobacterial infections Plant extracts Phytochemicals Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title | Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title_full | Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title_fullStr | Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title_full_unstemmed | Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title_short | Antibiofilm activity of South African plant extracts against Mycobacterium spp. and their mechanism of action using Mycothiol reductase |
| title_sort | antibiofilm activity of south african plant extracts against mycobacterium spp and their mechanism of action using mycothiol reductase |
| topic | UCTD Antibiofilm activity Biofilm inhibition Mycobacterium spp. Mycobacterial infections Plant extracts Phytochemicals Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/79237 |