Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos
Dissertation (MSc Agric Animal Science (Reproduction Physiology))--University of Pretoria,2020.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2021
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613610733207552 |
|---|---|
| access_status_str | Open Access |
| author2 | Maqhashu, Ayanda |
| author_browse | Maqhashu, Ayanda |
| author_facet | Maqhashu, Ayanda |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc Agric Animal Science (Reproduction Physiology))--University of Pretoria,2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/79882 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:53.515Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/79882 Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos Maqhashu, Ayanda u15091784@tuks.co.za Webb, E.C. (Edward Cottington) Mphaphathi, Masindi Honneysett, Kayla M. Reproduction Physiology Animal Science Porcine embryos Porcine oocytes Oocyte retrieval methods In vitro fertilization (IVF) In vitro maturation (IVM) Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 Dissertation (MSc Agric Animal Science (Reproduction Physiology))--University of Pretoria,2020. Oocyte recovery, in vitro maturation (IVM) and in vitro fertilization (IVF) are reproductive technologies used to advance an oocyte to an embryo stage. The objectives of the study were to (1), compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; (2), compare IVM media (NCSU 37, TCM 199 and mpFF= porcine follicular fluid + FSH + LH) on oocytes polar body extrusion; (3), compare the effect of electrical stimulation (ES) and frozen-thawed semen on cleavage rates and (4) compare the effect of culture media, cNCSU 37 and cpFF, on zygote cleavage rates. Follicular fluid collected from the retrieval methods were assessed for the presence of oocytes using a microscope and categorized as Grades A, B or C. The IVM media were placed in a four-well multidish, thereafter Grades A and B oocytes were randomly allocated per treatment groups, matured for 44 hours and checked for polar body extrusion. Matured ootids were randomly allocated to either ES treatment or IVF treatment with frozen-thawed semen. The ES treatment used a medium consisting mainly of mannitol while BO-IVF was used for the frozen-thawed treatment. ES and IVF took place in conjuncture with in vitro culture (IVC), where both results were obtained following placement in IVC media. Zygotes were then checked and recorded for cleavage, single cell or lysed. The treatment means were compared using the Fisher’s protected t-test least significant difference. Results showed significant differences with Grades A and B accounting for 50.8% of total oocytes (193.8) for aspiration and 58.7% of 488.6 for slicing. Oocytes’ polar body extrusion was recorded as 25.3%, 84.2% and 73.8% for NCSU 37, TCM 199 and mpFF respectively. The percentage of cleaved zygotes for IVF with frozen-thawed semen was 48.1% and 56.8% for ES. The percentage of cleaved zygotes for cNCSU 37 was 66.6% and was 45.7% for cpFF. The slicing method proved to be better than aspiration for the retrieval of Grades A and B oocytes and the total number of oocytes retrieved. TCM 199 and mpFF media had a higher percentage of oocytes with polar body extrusion than NCSU 37. Cleavage rate of zygotes was higher when using ES and cNCSU 37. Department of Agriculture (DAFF) Research and Development (R&D) Projects bs2026 Animal and Wildlife Sciences MSc Agric Animal Science (Reproduction Physiology) Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2021-05-13T10:30:38Z 2021-05-13T10:30:38Z 2021 2021 Dissertation * S2019 http://hdl.handle.net/2263/79882 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Reproduction Physiology Animal Science Porcine embryos Porcine oocytes Oocyte retrieval methods In vitro fertilization (IVF) In vitro maturation (IVM) Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title | Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title_full | Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title_fullStr | Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title_full_unstemmed | Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title_short | Influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| title_sort | influence of oocyte retrieval methods and maturation media on in vitro development of porcine embryos |
| topic | Reproduction Physiology Animal Science Porcine embryos Porcine oocytes Oocyte retrieval methods In vitro fertilization (IVF) In vitro maturation (IVM) Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-12 |
| url | http://hdl.handle.net/2263/79882 |