Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism
Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2020.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2021
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613465028329472 |
|---|---|
| access_status_str | Open Access |
| author2 | Botha, C.J. (Christoffel Jacobus) |
| author_browse | Botha, C.J. (Christoffel Jacobus) |
| author_facet | Botha, C.J. (Christoffel Jacobus) |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/80470 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:34.553Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/80470 Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism Botha, C.J. (Christoffel Jacobus) matheyvette@gmail.com Mathe, Yvette Zethu UCTD Geigeria aspera cytotoxicity ivalin geigerin isogeigerin acetate parthenolide microsomal fraction Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Dissertation (MSc (Veterinary Sciences))--University of Pretoria, 2020. Vermeersiekte or ‘vomiting disease’ is an economically important disease of ruminants following ingestion of Geigeria species in South Africa. Sheep are more susceptible and poisoning is characterised by stiffness, regurgitation, bloat, paresis and paralysis. Geigeria aspera was collected in the Vrede district (27° 25′ 48″ S; 29° 9′ 36″ E), Free State Province. The plant material was dried, milled and the toxic principles, known as sesquiterpene lactones, were extracted and isolated following chromatographic procedures. Even though geigerin and ivalin were previously isolated, an unknown sesquiterpene lactone, isogeigerin acetate, was also purified. Mouse myoblast (C2C12) and rat embryonic cardiac myocyte (H9c2) cell lines were exposed to different concentrations of geigerin, ivalin, isogeigerin acetate and a commercially available sesquiterpene lactone, parthenolide. An in vitro colorimetric assay 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to assess cytotoxicity. The median effective concentrations (EC50) indicated that ivalin and parthenolide were significantly (p<0.05) more toxic than geigerin and isogeigerin acetate. A concentration-dependent cytotoxic response was observed in both cell lines, however, C2C12 cells were more sensitive. A high performance liquid chromatography (HPLC) analysis was used to evaluate the in vitro metabolism of parthenolide, following the addition of a mouse liver microsomal fraction. Results revealed that parthenolide, incubated with the microsomal fraction, undergoes enzymatic transformation to form a metabolite. pt2021 es2026 Paraclinical Sciences MSc (Veterinary Sciences) Unrestricted SDG-02: Zero hunger SDG-03: Good health and well-being SDG-12: Responsible consumption and production 2021-06-22T12:29:13Z 2021-06-22T12:29:13Z 2020/10/01 2020 Dissertation Mathe, YZ 2020, Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism, MSc (Veterinary Sciences) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/80470> S2020 http://hdl.handle.net/2263/80470 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Geigeria aspera cytotoxicity ivalin geigerin isogeigerin acetate parthenolide microsomal fraction Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title | Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title_full | Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title_fullStr | Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title_full_unstemmed | Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title_short | Sesquiterpene lactones from Geigeria aspera : isolation, cytotoxicity against murine muscle cell lines and microsomal metabolism |
| title_sort | sesquiterpene lactones from geigeria aspera isolation cytotoxicity against murine muscle cell lines and microsomal metabolism |
| topic | UCTD Geigeria aspera cytotoxicity ivalin geigerin isogeigerin acetate parthenolide microsomal fraction Veterinary science theses SDG-02 Veterinary science theses SDG-03 Veterinary science theses SDG-12 |
| url | http://hdl.handle.net/2263/80470 |