Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs
Dissertation (MSc (Microbiology))--University of Pretoria, 2021.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2021
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613644158664705 |
|---|---|
| access_status_str | Open Access |
| author2 | Nel, Louis Hendrik |
| author_browse | Nel, Louis Hendrik |
| author_facet | Nel, Louis Hendrik |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/81511 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:25.358Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/81511 Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs Nel, Louis Hendrik u15062555@tuks.co.za Wright, Nicolette Arnold, Danielle Patricia Dog population management towards rabies control Dissertation (MSc (Microbiology))--University of Pretoria, 2021. Rabies is a viral disease caused by the rabies lyssavirus (RABV). Despite effective rabies vaccines for humans and animals, this disease continues to pose a major public health challenge, causing an estimated 59 000 human deaths each year, over 99% of which are caused by the domestic dog (Canis familiaris). Current methods of dog population management used in rabies control programs are ineffective. Surgical sterilisation does not reach enough of the dog population to curb population densities and contraceptives need to be administered at a specific phase in the oestrous cycle or cause a range of side effects. Immunocontraception in dogs would allow rabies vaccination coverage to be maintained, in turn reducing the burden of rabies on public health. The aim of this study was to develop an immunocontraceptive vaccine for dogs capable of eliciting a stronger immune response than that of previously constructed vaccines allowing for effective dog population management and allowing rabies vaccination coverage to be maintain, in turn reducing the burden of rabies on public health. By stabilising the dog population size, the 70% vaccination coverage required to interrupt rabies transmission within a population can be maintained. The immunocontraceptive vaccine constructed in this study contained two reproductive hormones, namely GnRH and kisspeptin, in the hope of eliciting a stronger contraceptive effect than either of these could produce alone, as well as the partial tetanus toxoid gene as an immune stimulant. The nucleic acid GnRH, kisspeptin and partial tetanus toxoid gene (GKT) insert fragment was PCR amplified from a DNA construct (pVAC-GKT) and was cloned into the adenoviral vector using In-fusion cloning technology. Transfection of pAdeno-X 293 cells was confirmed using green fluorescent microscopy and expression of the Ad-GKT mRNA in cell culture was confirmed using real-time RT-PCR. The antigenicity of the Ad-GKT construct was evaluated using female Swiss Webster mice. An indirect ELISA was used to detect seroconversion of the GnRH and Kisspeptin insert fragments. The Ad-GKT construct was successful in eliciting an immune response against GnRH and kisspeptin. Future research should include a comparative study to determine the antigenicity of the Ad-GnRH1 and Ad-GKT constructs in a canine trial for potential use in rabies control programs. The Poliomyelitis Research Foundation grant (19/89). University of Pretoria postgraduate masters research bursary. Technology Innovation Agency Seed Fund. National Research Foundation grant (122016). Microbiology and Plant Pathology MSc (Microbiology) Restricted 2021-08-26T12:47:31Z 2021-08-26T12:47:31Z 2022-04 2021 Dissertation * S2021 http://hdl.handle.net/2263/81511 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Dog population management towards rabies control Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title | Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title_full | Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title_fullStr | Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title_full_unstemmed | Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title_short | Development of a recombinant adenoviral immunocontraceptive vaccine (Ad-GKT) for use in domestic dogs |
| title_sort | development of a recombinant adenoviral immunocontraceptive vaccine ad gkt for use in domestic dogs |
| topic | Dog population management towards rabies control |
| url | http://hdl.handle.net/2263/81511 |