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Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees
Dissertation (MSc (Biotechnology))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2021
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| _version_ | 1867613710875361280 |
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| access_status_str | Open Access |
| author2 | Maloney, Victoria |
| author_browse | Maloney, Victoria |
| author_facet | Maloney, Victoria |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biotechnology))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/82921 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:28.830Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/82921 Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees Maloney, Victoria nganea.nangammbi@up.ac.za Myburg, Alexander Andrew Mizrachi, Eshchar Nangammbi, Nganeavhutshilo Functional genetics UCTD Dissertation (MSc (Biotechnology))--University of Pretoria, 2021. On a quest to eliminate the use of fossil fuels to alleviate challenges associated with climate change, it has become vital to rely on lignocellulosic biomass as an alternative source for generating value-added products and sustaining the bioeconomy. However, the structure of lignocellulosic biomass contributes to biomass recalcitrance, necessitating genetic engineering of plant cell walls for easier and efficient bioconversion. The success of genetic engineering approach requires a significant understanding of the function and biological roles of cell wall biosynthetic genes. Here, we functionally analysed the impact of knockout mutation of several xylan-associated genes on plant growth and physicochemical properties. We established that XYLANASE 1(XYN1) may be necessary for water and nutrient transport in Arabidopsis thaliana, as xyn1 T-DNA insertional mutants had significantly shorter roots and leaves. More importantly, we show that the Arabidopsis EMP70 is essential during seed germination and mutation thereof leads to early germination and subsequent vegetative growth advantage. Realizing the exact biological roles of these and other novel cell wall biosynthetic genes will be beneficial in understanding the development of cell wall in energy crops such as Eucalyptus, and in future, it may contribute to improving bioprocessing efficiency while reducing the associated bioprocessing costs. Sappi NRF DST TIA Genetics MSc (Biotechnology) Unrestricted 2021-12-01T09:02:25Z 2021-12-01T09:02:25Z 2022-05-01 2021-11-30 Dissertation Nangammbi, N 2021, Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees, MSc dissertation, University of Pretoria, Pretoria http://hdl.handle.net/2263/82921 A2022 http://hdl.handle.net/2263/82921 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Functional genetics UCTD Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title | Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title_full | Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title_fullStr | Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title_full_unstemmed | Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title_short | Functional testing of xylan-associated genes in Arabidopsis and application of CRISPR/Cas9 gene editing in hybrid poplar trees |
| title_sort | functional testing of xylan associated genes in arabidopsis and application of crispr cas9 gene editing in hybrid poplar trees |
| topic | Functional genetics UCTD |
| url | http://hdl.handle.net/2263/82921 |