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Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis
Dissertation (MMedVet (Clinical Laboratory Diagnostics))--University of Pretoria, 2020.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613614035173376 |
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| access_status_str | Open Access |
| author2 | Goddard, Amelia |
| author_browse | Goddard, Amelia |
| author_facet | Goddard, Amelia |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MMedVet (Clinical Laboratory Diagnostics))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83215 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:56.612Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83215 Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis Goddard, Amelia u27177450@tuks.co.za Rautenbach, Yolandi Du Preez, Kelly UCTD Dissertation (MMedVet (Clinical Laboratory Diagnostics))--University of Pretoria, 2020. Canine parvoviral enteritis (CPE) is a serious disease characterised by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. The aim of this study was to characterise the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Blood was collected from 28 dogs with CPE and 11 healthy, age-matched, control dogs. Oxidative burst activity was assessed by stimulating phagocytes with opsonised Escherichia coli or phorbol 12-myristate 13-acetate (PMA) and measuring the percentage of phagocytes producing reactive oxygen species and the magnitude of this production. Similarly, phagocytosis was measured by incubating phagocytes with opsonised E. coli and measuring the percentage of phagocytes containing E. coli and the number of bacteria per cell. Complete blood counts and serum C-reactive protein (CRP) concentrations were also determined. Serum CRP concentration was negatively and positively correlated with segmented and band neutrophil concentrations, respectively. Overall, no differences in phagocyte function were found between the dogs with CPE and healthy control dogs. However, infected dogs with neutropenia or circulating band neutrophils had decreased PMA-stimulated oxidative burst activity compared to healthy controls. Additionally, CPE dogs with neutropenia or circulating band neutrophils showed decreased PMA- and E. coli-stimulated oxidative burst activity and decreased phagocytosis of E. coli compared to CPE dogs without neutropenia or band neutrophils. In conclusion, there is evidence that canine phagocytes have decreased oxidative burst and phagocytic activity in neutropenic CPE dogs and CPE dogs with circulating band neutrophils. Companion Animal Clinical Studies MMedVet (Clinical Laboratory Diagnostics) Unrestricted 2022-01-12T06:00:19Z 2022-01-12T06:00:19Z 2021/04/16 2020 Dissertation * A2021 http://hdl.handle.net/2263/83215 DOI: 10.25403/UPresearchdata.13090247 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title | Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title_full | Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title_fullStr | Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title_full_unstemmed | Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title_short | Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| title_sort | oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis |
| topic | UCTD |
| url | http://hdl.handle.net/2263/83215 |