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Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613567638831104 |
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| access_status_str | Open Access |
| author2 | Leisewitz, Andrew L. |
| author_browse | Leisewitz, Andrew L. |
| author_facet | Leisewitz, Andrew L. |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83288 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:12.302Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83288 Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa Leisewitz, Andrew L. u29072299@tuks.co.za Karama, Musafiri Moodley, Arshnee Prior, Cameron David UCTD Dissertation (MSc (Veterinary Science))--University of Pretoria, 2021. Staphylococcus pseudintermedius is an important opportunistic commensal bacterium, often correlated with dermatitis and otitis in small animals. The emergence and rapid expansion of methicillin resistance giving rise to methicillin-resistant S. pseudintermedius (MRSP) is of concern as it is often correlated with multi-drug resistance, thereby reducing therapeutic options for these common veterinary conditions. The study aims to 1) evaluate standard laboratory methods used at five regional veterinary laboratories in South Africa to identify S. pseudintermedius; 2) determine if an association exists between resistance to first and second tier antibiotics and the presence of the mecA gene; and 3) determine if there is an association between MRSPcarriage and previous antibiotic usage. Sixty-eight presumptive MRSP clinical samples from five geographically dispersed laboratories in Republic of South Africa (RSA) were collected over a 24-month period. S. pseudintermedius was detected by a standard laboratory method and antimicrobial susceptibility testing done by means of disc diffusion. Presumptive MRSP isolates were identified when disc diffusion showed resistance to oxacillin. PCR confirmed MRSA clinical isolates by the presence of mecA. Fifty-seven samples were confirmed to harbour S. pseudintermedius (83.8%) and 49 (85%) of those were further identified to carry mecA. Of the 49 mecA positive PCR isolates, 28 were isolated from pruritic patients (28/49, 57%) and 7/49 from otitis (14%).This study provides evidence that there is a high prevalence of mecA positive carriage (85% of samples) in methicillin resistant SP pyoderma and otitis in dogs in South Africa. Important risk factors for mecA positive carriage are previous hospital admission, pruritis and previous antibacterial failure. Thus, the data suggests that there is an urgent need for better surveillance of dogs presenting with pyoderma and otitis in South Africa. Moreover, diligent antibiotic stewardship will be crucial to prevent a deterioration of this situation in the country. Companion Animal Clinical Studies MSc (Veterinary Science) Unrestricted 2022-01-12T06:01:22Z 2022-01-12T06:01:22Z 2021/04/16 2021 Dissertation * A2021 http://hdl.handle.net/2263/83288 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title | Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title_full | Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title_fullStr | Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title_full_unstemmed | Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title_short | Prevalence of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs with skin and ear infections in South Africa |
| title_sort | prevalence of methicillin resistance in staphylococcus pseudintermedius isolates from dogs with skin and ear infections in south africa |
| topic | UCTD |
| url | http://hdl.handle.net/2263/83288 |