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Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells
Dissertation (MSc (Medical Immunology))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613502232854528 |
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| access_status_str | Open Access |
| author2 | Pepper, Michael Sean |
| author_browse | Pepper, Michael Sean |
| author_facet | Pepper, Michael Sean |
| collection | Thesis |
| dc_rights_str_mv | © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Immunology))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83389 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:10.039Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83389 Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells Pepper, Michael Sean aurona.rohmien@gmail.com Gerber, Aurona Jacoba Medical Immunology UCTD Dissertation (MSc (Medical Immunology))--University of Pretoria, 2021. The use of human adipose derived stem/stromal cells (hASCs) in a therapeutic setting is has increased research in the field with various clinical trials currently being conducted (REF).However, the use of foetal bovine serum (FBS) as a standard hASC culture media supplement poses challenges towards a good manufacturing practices (GMP) compliant therapeutic hASC product. Human blood component alternatives to FBS for cell culture serum supplementation were investigated for compliance to good manufacturing practices (GMP) when considering hASCs for therapeutic use. A head-to-head comparison of five human alternatives to FBS for hASC culture medium supplementation was completed. hASCs were expanded in five human alternatives and FBS, and the morphology, proliferation, viability, and retention of adipogenic potential of ASCs were investigated and compared. All human alternatives resulted in faster proliferation compared to FBS. Pooled human platelet lysate (pHPL) and platelet-rich-plasma (PRP) were identified as the best alternatives for hASC expansion in vitro as they resulted in faster hASC proliferation than human serum (HS), fresh-frozen plasma (FFP), platelet-poor plasma (PPP), and FBS. hASCs were able to differentiate into adipocytes in pHPL and PRP (the two human alternatives resulting in the fastest proliferation) and FBS. The study results lead to a conclusion that it is possible to use human alternatives to FBS for in vitro hASC expansion as the first step towards producing a GMP compliant hASC product for use in regenerative medicine and cellular therapy approaches. NRF Immunology MSc (Medical Immunology) Unrestricted 2022-01-19T07:48:51Z 2022-01-19T07:48:51Z 2022-04 2021 Dissertation Gerber AR 2021, Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd http://hdl.handle.net/2263/83389 http://hdl.handle.net/2263/83389 en © 2019 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Medical Immunology UCTD Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title | Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title_full | Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title_fullStr | Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title_full_unstemmed | Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title_short | Human alternatives to foetal bovine serum for the expansion of human adipose-derived stem cells |
| title_sort | human alternatives to foetal bovine serum for the expansion of human adipose derived stem cells |
| topic | Medical Immunology UCTD |
| url | http://hdl.handle.net/2263/83389 |