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The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts
Dissertation (MSc (Microbiology))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613662327341056 |
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| access_status_str | Open Access |
| author2 | Hammerbacher, Almuth |
| author_browse | Hammerbacher, Almuth |
| author_facet | Hammerbacher, Almuth |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83527 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:42.666Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83527 The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts Hammerbacher, Almuth rosa.knoppersen@up.ac.za Coutinho, Teresa A. Knoppersen, Rosa Sophie Microbiology Entomology Gut Microbiome UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2021. The Eucalyptus snout beetle is an oligophagous invasive insect pest of Eucalyptus species. The beetle is attracted to emerging leaves containing high concentrations of essential oils consisting of a complex mixture of mono and sesquiterpenes. In Eucalyptus, terpenoids can function as insect repellents through powerful odours and bitter taste. Furthermore, on a molecular scale, terpenoids are involved in breaking down cell membranes and negatively affecting ion transport. Regardless of the elaborate chemical defences of Eucalyptus species, the Eucalyptus snout beetle is able to tolerate and overcome high concentrations of terpenoids. We, therefore, hypothesize that the microbial communities associated with the gut of Gonipterus spp. are involved in secondary metabolite detoxification. Gonipterus sp. n. 2 adults were reared on two Eucalyptus genotypes with significantly different biochemical profiles. Metabolomic analysis of the leaf and beetle frass profiles indicated that there was a dramatic decrease in terpenoid concentrations from leaf to frass. Moreover, several biotransformation products of eucalyptol, α-pinene and d-limonene were found in the frass, suggesting that Gonipterus sp. n. 2 gut microbial communities may facilitate in Eucalyptus secondary metabolite detoxification. Following this discovery, a 16s rRNA gene sequencing approach was used to investigate the bacterial diversity of the host plants, gut and frass of Gonipterus sp. n. 2. Additionally, to determine if the Gonipterus gut microbial communities detoxified Eucalyptus secondary metabolites in vivo, an attempt was made to disrupt the gut bacterial community through antibiotic treatment. Sequencing results indicated that plant bacterial communities were enriched with Clostridiales and Sphingomonadales whereas gut and frass associated bacterial communities were dominated by Enterobacteriales. Furthermore, a core gut microbial community was detected, with a large percentage of the bacterial families shared between the Gonipterus beetle groups despite diet differences. Antibiotic treatment failed to disrupt the gut microbial communities of Gonipterus sp. n. 2, as no significant difference was observed between antibiotic and control beetle treatments. Therefore, more research is required to determine the role of Gonipterus gut microbial communities in detoxifying Eucalyptus secondary metabolites International Centre for Genetic Engineering and Biotechnology (ICGEB) Tree Protection Co-operative Programme (TPCP) Microbiology and Plant Pathology MSc (Microbiology) Unrestricted 2022-01-31T09:04:45Z 2022-01-31T09:04:45Z 2022-04 2021 Dissertation Knoppersen, RS 2021, The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd http://hdl.handle.net/2263/83527 A2022 http://hdl.handle.net/2263/83527 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Microbiology Entomology Gut Microbiome UCTD The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title | The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title_full | The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title_fullStr | The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title_full_unstemmed | The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title_short | The bacterial diversity associated with the gut of Gonipterus sp.n.2 fed on two different Eucalyptus hosts |
| title_sort | bacterial diversity associated with the gut of gonipterus sp n 2 fed on two different eucalyptus hosts |
| topic | Microbiology Entomology Gut Microbiome UCTD |
| url | http://hdl.handle.net/2263/83527 |