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The immune response of Sirex noctilio to pathogen infection
Dissertation (MSc (Genetics))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613502381752320 |
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| access_status_str | Open Access |
| author2 | Slippers, Bernard |
| author_browse | Slippers, Bernard |
| author_facet | Slippers, Bernard |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83791 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:10.039Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83791 The immune response of Sirex noctilio to pathogen infection Slippers, Bernard u10166760@tuks.co.za Postma, Alisa Makua, Malehlogonolo Sophie UCTD Insect immune system Dissertation (MSc (Genetics))--University of Pretoria, 2021. The woodwasp, Sirex noctilio, in association with its mutualistic fungus, Amylostereum areolatum, pose a threat to forest plantations in the Southern Hemisphere. This invasive pest insect is controlled with a biological control agent, a parasitic nematode, Deladenus siricidicola. This biological control method has previously been successful in controlling S. noctilio populations, achieving over 90% parasitism. Recent studies have shown that there is a variability in the efficiency of the nematode and there are some S. noctilio populations that are resistant to certain nematode strains. A better understanding of the immune response of the wasp during its interaction with the nematode has relevance to the evolution of immune response pathways in Hymenoptera in general and may also assist in the selection of more virulent/effective nematode strains in the future. The molecular mechanisms underlying the interaction between S. noctilio and D. siricidicola are, however, unknown. In this study, we aimed to identify the immune-related genes of this wasp. To enhance our findings, we used transcriptome analysis on immune challenged and unchallenged S. noctilio larvae to identify additional components implicated in immune reactions. A total of 180 immune-related genes were identified through the comparison of the S. noctilio genome with the genes and genomes of other Hymenoptera. Key elements of the conserved Toll, IMD, JNK and JAK-STAT signalling pathways were identified in the S. noctilio genome. Differential gene expression analyses performed on infected S. noctilio larvae demonstrated that typical wounding response mechanisms are activated by this insect in response to all the treatments. This study provides insight into the molecular pathways of innate immune processes in S. noctilio larvae and will serve as the foundation of future studies on the interaction between the woodwasp and pathogens/parasites. NRF FABI Genetics MSc (Genetics) Unrestricted 2022-02-11T08:10:35Z 2022-02-11T08:10:35Z 2022-04 2021 Dissertation Makua, MS 2021, The immune response of Sirex noctilio to pathogen infection, MSc dessertation, University of Pretoria A2022 http://hdl.handle.net/2263/83791 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Insect immune system The immune response of Sirex noctilio to pathogen infection |
| title | The immune response of Sirex noctilio to pathogen infection |
| title_full | The immune response of Sirex noctilio to pathogen infection |
| title_fullStr | The immune response of Sirex noctilio to pathogen infection |
| title_full_unstemmed | The immune response of Sirex noctilio to pathogen infection |
| title_short | The immune response of Sirex noctilio to pathogen infection |
| title_sort | immune response of sirex noctilio to pathogen infection |
| topic | UCTD Insect immune system |
| url | http://hdl.handle.net/2263/83791 |