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Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia
Dissertation (MSc (Microbiology))--University of Pretoria, 2021.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613695747555328 |
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| access_status_str | Open Access |
| author2 | Barnes, Irene |
| author_browse | Barnes, Irene |
| author_facet | Barnes, Irene |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/83894 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:14.504Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/83894 Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia Barnes, Irene grannyjaynet95@gmail.com Wingfield, Michael J. Roux, Jolanda Hlongwane, Granny UCTD Microbiology Tree Protection Co-operative Programme (TPCP) Ceratocystis infections Plantation-grown trees Dissertation (MSc (Microbiology))--University of Pretoria, 2021. Ceratocystis infections pose significant threats to the sustainability of plantation-grown trees propagated for commercial purposes. Ceratocystis infections result in wilt, canker, vascular discolourations and eventual mortality of the host species. Symptoms typical of Ceratocystis infections have recently emerged on Eucalyptus spp. in Indonesia and South Africa. The aims of this study were to characterize Ceratocystis isolates collected from diseased Eucalyptus spp. in South Africa and those from Eucalyptus spp. and Acacia spp. in Indonesia, and also determine their population genetic diversity, population structure and their level of aggressiveness towards healthy Eucalyptus spp. For the South African population, isolates previously collected from heathy, but wounded Eucalyptus, were included for comparative purposes. Results of sequence data and multi-gene phylogenetic analyses revealed that C. manginecans and C. eucalypticola are the pathogens infecting Eucalyptus spp. in Indonesia and South Africa, respectively. In Indonesia, a greater genetic diversity was found in isolates from Acacia than those from Eucalyptus. This, together with the presence of the same multilocus haplotypes on both species corresponds with a longer history of infection on Acacia spp. and a recent host expansion onto Eucalyptus. On the contrary, in South Africa, isolates from wounds on healthy Eucalyptus trees and those from the diseased trees, as well as soil had low levels of diversity and lacked population structure. Interestingly, the same multi-locus haplotypes were found in both the non-diseased and diseased populations which shows that their isolates represent the same species. Therefore, the recent emergence of Ceratocystis disease outbreak on Eucalyptus spp. in South Africa seem to be associated with the susceptibility of the recently propagated Eucalyptus spp. Field inoculation tests showed that Acacia and Eucalyptus isolates collected in Indonesia comprise of a mixture of aggressive and non-aggressive individuals. On the contrary, inoculations tests done using isolates of C. eucalypticola described in the absence of a disease and those that recently emerged as causal agents of Eucalyptus wilt in South Africa resulted in similar aggressiveness levels. Results of this study have expanded current knowledge on Ceratocystis species by adding substantial information regarding their distribution and genetic diversity. They also highlight the need for the selection of resistant host species to help reduce plantation-grown tree mortality. Tree Protection Co-operative Programme (TPCP), the Department of Science and Innovation (DSI)/ National Research Foundation (NRF) Centre of Excellence in Plant Health Biotechnology (CPHB) as well as SAPPI Ltd, South Africa. Forestry and Agricultural Biotechnology Institute (FABI) MSc (Microbiology) Unrestricted 2022-02-14T13:46:17Z 2022-02-14T13:46:17Z 2022 2021 Dissertation Hlongwane, G 2021, Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia, MSc thesis, University of Pretoria, Pretoria viewed yyyymmdd http://hdl.handle.net/2263/83894 A2022 http://hdl.handle.net/2263/83894 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Microbiology Tree Protection Co-operative Programme (TPCP) Ceratocystis infections Plantation-grown trees Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title | Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title_full | Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title_fullStr | Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title_full_unstemmed | Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title_short | Identification and population genetic studies on Ceratocystis spp. infecting Eucalyptus and Acacia plantations in South Africa and Indonesia |
| title_sort | identification and population genetic studies on ceratocystis spp infecting eucalyptus and acacia plantations in south africa and indonesia |
| topic | UCTD Microbiology Tree Protection Co-operative Programme (TPCP) Ceratocystis infections Plantation-grown trees |
| url | http://hdl.handle.net/2263/83894 |