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Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils

Dissertation (MSc)--University of Pretoria, 2014.

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Other Authors: Tesfamariam, Eyob Habte
Format: Thesis
Language:English
Published: University of Pretoria 2022
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access_status_str Open Access
author2 Tesfamariam, Eyob Habte
author_browse Tesfamariam, Eyob Habte
author_facet Tesfamariam, Eyob Habte
collection Thesis
dc_rights_str_mv © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
description Dissertation (MSc)--University of Pretoria, 2014.
format Thesis
id oai:repository.up.ac.za:2263/84065
institution University of Pretoria (South Africa)
language English
last_indexed 2026-06-10T12:40:08.409Z
license_str Other — see source repository
provenance_str_mv Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository
publishDate 2022
publishDateRange 2022
publishDateSort 2022
publisher University of Pretoria
publisherStr University of Pretoria
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source_str UPSpace — University of Pretoria Institutional Repository
spelling oai:repository.up.ac.za:2263/84065 Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils Tesfamariam, Eyob Habte euob.tesfamariam@up.ac.za De Jager, Pieter Christiaan Malobane, Mashapa Elvis Sludge Lignified fraction Cellulose Hemicellulose Lipids Soluble compounds N and C UCTD Dissertation (MSc)--University of Pretoria, 2014. The composition of sludge organic matter is mainly influenced by the origin of wastewater. The biochemical composition of sludge could, however, alter from post wastewater treatment drying techniques. Such changes have a direct effect on the N fertilizer value of sludge because the biochemical composition of sludge dictates its decomposition rate. Therefore, proper understanding of the effect of wastewater treatment methods and post treatment drying techniques on a) the C and N contents as well as organic matter composition of sludge, and b) C decomposition and N mineralization is crucial for N management in sludge amended agricultural lands. The aim of this study was to investigate the effect of selected wastewater treatment and post treatment drying techniques on a) the N, C, and organic matter composition, as well as b) C decomposition and N mineralization of municipal sewage sludge. The study also investigated the compounds responsible for C and N release during a decomposition process. Two consecutive incubation studies (100 days each) were conducted to determine N release, C decomposition and sludge organic matter decomposition using different sludge types namely: Thermally hydrolysed sludge (THS), Activated sludge (Activated), and anaerobically digested sludge. The anaerobically digested sludges were collected from two different drying techniques (concrete beds and earth paddy) as well as two sludge drying depths on beds ( 10 cm and 25 cm). Inorganic N was determined by the steam distillation method. Sludge organic matter composition was analysed using Van Soest method. Total N and total organic C were determined using Carlo-Erba method. A parallel sludge alone incubation study was conducted from which organic compounds degradation and transformation analysis were conducted. The anaerobically digested sludge dried in thin layers of 10 cm (ADS3) had higher total N (4.95%) than similar sludge types dried in thick layers of 25 cm in concrete beds (ADS1= 2.81%N) and in earth paddy (ADS2 = 2.83%N). Large fraction (>60%) of the organic C in all sludge types was in soluble compounds form (SOL). Thermally hydrolyzed sludge (THS) had the highest (90%) SOL. ADS3 and Activated had lower lignin fraction (<3%) than THS, ADS1 and ADS2. Net N mineralization per kg C applied was highest for activated (77 g kg-1 C) and was lowest for ADS2 (23 g kg-1 C). Despite having highest SOL, THS had relatively lower net N mineralization per kg organic N applied (350 g kg-1 organic N) than Activated sludge (449 g kg-1 organic N) mainly due to the relatively higher lignin fraction. It was apparent from this study that the soluble compounds were responsible for about 90% of the organic matter decomposition. The Van Soest method was also successfully used to determine residual compounds at intervals during the incubation study from the sludge alone study but not with the soilsludge mixture. National Research Foundation (NRF) Water Research Commission (WRC), East Rand Water Care Company (ERWAT) Plant Production and Soil Science MSc Unrestricted 2022-02-18T08:31:36Z 2022-02-18T08:31:36Z 2014 2014-11 Dissertation * http://hdl.handle.net/2263/84065 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria
spellingShingle Sludge
Lignified fraction
Cellulose
Hemicellulose
Lipids
Soluble compounds
N and C
UCTD
Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title_full Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title_fullStr Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title_full_unstemmed Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title_short Using the organic carbon fractions of the Van soest method to determine compounds responsible for C and N mineralization from sludge amended soils
title_sort using the organic carbon fractions of the van soest method to determine compounds responsible for c and n mineralization from sludge amended soils
topic Sludge
Lignified fraction
Cellulose
Hemicellulose
Lipids
Soluble compounds
N and C
UCTD
url http://hdl.handle.net/2263/84065