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Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates
Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2015.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613453736214528 |
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| access_status_str | Open Access |
| author2 | Kock, Martha Magdalena |
| author_browse | Kock, Martha Magdalena |
| author_facet | Kock, Martha Magdalena |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/84069 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:23.737Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/84069 Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates Kock, Martha Magdalena marleen.kock@up.ac.za Ehlers, M.M. (Marthie Magdaleen) De Jesus, Marissa Batista Klebsiella pneumoniae β-Lactamases Pulsed-field gel electrophoresis Multilocus sequence typing UCTD Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-17 SDG-17: Partnerships for the goals Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2015. Genetic typing of Klebsiella pneumoniae is used for epidemiological referencing. In the clinical setting it can be useful in outbreak investigations, understanding transmission and managing hospital infections. Multi-drug resistant bacteria exist and proliferate either due to natural selection of clonal lineages or the transfer of mobile genetic elements, sometimes in response to antibiotic-use selective pressure. Pulsed-field gel electrophoresis (PFGE) is highly discriminatory and the gold standard typing method for the characterisation of K. pneumoniae isolates. The aim of the study was to genetically characterise K. pneumoniae isolates by PFGE and multilocus sequence typing (MLST). One hundred unrepeated ESBL-producing K. pneumoniae isolates were collected from the National Health Laboratory Service (NHLS). The PFGE was performed on a Rotaphor VI system (Biometra, Germany). Clonal representatives were further characterised by MLST. All the strains were typeable by PFGE using XbaI, which discerned multiple pulsotypes and MLST identified 10 different STs including a novel sequence type, ST1632. The diverse pulsotypes of K. pneumoniae isolates are not suggestive of clonal spread of particular strains. The MLST results further confirmed the variability among isolates tested and elucidated several STs, some of which have been identified internationally and often associated with carbapenem-resistance. Data on K. pneumoniae STs is still limited in the South African clinical setting, although the close monitoring of resistance profiles and characterisation of isolates is imperative for outbreak analysis, identification of prominent STs in clinical settings as compared to international counterparts and surveillance of expanding resistance. em2025 Medical Microbiology MSc (Medical Microbiology) Unrestricted SDG-03: Good health and well-being SDG-17: Partnerships for the goals 2022-02-18T08:49:58Z 2022-02-18T08:49:58Z 2015 2015 Dissertation * http://hdl.handle.net/2263/84069 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Klebsiella pneumoniae β-Lactamases Pulsed-field gel electrophoresis Multilocus sequence typing UCTD Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-17 SDG-17: Partnerships for the goals Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title | Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title_full | Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title_fullStr | Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title_full_unstemmed | Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title_short | Molecular characterisation of β-lactamase producing Klebsiella pneumoniae isolates |
| title_sort | molecular characterisation of β lactamase producing klebsiella pneumoniae isolates |
| topic | Klebsiella pneumoniae β-Lactamases Pulsed-field gel electrophoresis Multilocus sequence typing UCTD Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-17 SDG-17: Partnerships for the goals |
| url | http://hdl.handle.net/2263/84069 |