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Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae)
Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2022.
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| Format: | Thesis |
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University of Pretoria
2022
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| _version_ | 1867613580206014464 |
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| access_status_str | Open Access |
| author2 | Bapela, Mahwahwatse Johanna |
| author_browse | Bapela, Mahwahwatse Johanna |
| author_facet | Bapela, Mahwahwatse Johanna |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/84251 |
| institution | University of Pretoria (South Africa) |
| language | en_US |
| last_indexed | 2026-06-10T12:38:24.243Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/84251 Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) Bapela, Mahwahwatse Johanna u13082176@tuks.co.za Mabuza, Mcebisi Junior Ethnobotany and ethnopharmacology UCTD Pappea capensis Antiplasmodial activity Medicinal plants Plant extracts Plasmodium Natural products Phytochemical screening Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2022. Ethnopharmacological relevance: Pappea capensis (Sapindaceae), traditionally used by VhaVenda people in South Africa to treat malaria, was evaluated for antiplasmodial activity and cytotoxicity. Materials and methods: Ground twigs were extracted using dichloromethane (DCM): 50% methanol (MeOH) (1:1, v/v), separated and dried under vacuum to yield DCM (I) and aqueous (II) extracts. The extract I was further partitioned using DCM: MeOH (1:1, v/v), separated and concentrated under vacuum to yield dichloromethane (III) and methanol (IV) crude extracts. A water-based decoction (V) was also prepared to establish the clinical relevance of the preparation administered by indigenous people. In vitro, antiplasmodial tests using the chloroquine-sensitive strain of Plasmodium falciparum (NF54) were conducted for all crude extracts (I – V). Cytotoxicity screening was performed on mammalian L-6 rat skeletal myoblast cells, and the plant samples' selectivity indices (SI) were computed. The crude extracts were then subjected to 1H NMR spectroscopy and GC-MS analysis to identify the major classes of compounds present in the crude extracts. Results and discussion: From the assayed crude extracts, I (IC50 = 2.93 μg /ml; SI = 14), III (IC50 = 2.59 μg /ml; SI = 21) and IV (IC50 = 3.56 μg /ml; SI = 13) demonstrated the best antiplasmodial activity and selectivity. Of all assayed fractions, only N (0.6 μg /ml; SI=91), D (0.85 μg /ml; SI=37) and E (0.91 μg /ml; SI =30) depicted the best antiplasmodial activity and selectivity. The 1H NMR analysis of crude extracts tentatively identified the prominent class of constituents to be aliphatic based. When subjected to GC-MS analysis, lupin-3-one, lupeol acetate, α-amyrin, and β-amyrin phytoconstituents were observed in the crude extracts. These identified compounds are (aliphatic-based constituents) with established antiplasmodial activity, and the observed antiplasmodial activity of P. capensis can be attributed to them. The results obtained from the GC-MS and 1H NMR data can be correlated with the observed antiplasmodial biological activity of P. capensis crude extracts. Conclusion: The study validates the ethnomedicinal use of P. capensis for malaria treatment. It demonstrated the potential of discovering novel antiplasmodial constituents that could serve as drug hits through dereplication approaches were known compounds with established antimalarial activity can be bypassed to focus on the unknown. 1. National Research Foundation (NRF) 2. University of Pretoria Institute for Sustainable Malaria Control (UP ISMC) bs2026 Plant Science MSc (Medicinal Plant Science) Unrestricted SDG-03: Good health and well-being SDG-09: Industry, innovation and infrastructure 2022-02-28T08:01:05Z 2022-02-28T08:01:05Z 2022 2022 Dissertation *Mabuza, MJ 2012, Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae), MSc dissertation, University of Pretoria, Pretoria, viewed 2022/02/27http://hdl.handle.net/2263/31804 A2022 http://hdl.handle.net/2263/84251 en_US © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Ethnobotany and ethnopharmacology UCTD Pappea capensis Antiplasmodial activity Medicinal plants Plant extracts Plasmodium Natural products Phytochemical screening Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title | Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title_full | Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title_fullStr | Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title_full_unstemmed | Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title_short | Antiplasmodial properties of extracts from Pappea capensis Eckl. & Zeyh. (Sapindaceae) |
| title_sort | antiplasmodial properties of extracts from pappea capensis eckl zeyh sapindaceae |
| topic | Ethnobotany and ethnopharmacology UCTD Pappea capensis Antiplasmodial activity Medicinal plants Plant extracts Plasmodium Natural products Phytochemical screening Natural and agricultural sciences theses SDG-03 Natural and agricultural sciences theses SDG-09 |
| url | http://hdl.handle.net/2263/84251 |