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The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal
Thesis (DSc)--University of Pretoria, 1984.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613688038424576 |
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| access_status_str | Open Access |
| author2 | Steyn, P.L. |
| author_browse | Steyn, P.L. |
| author_facet | Steyn, P.L. |
| collection | Thesis |
| dc_rights_str_mv | © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (DSc)--University of Pretoria, 1984. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/85493 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:07.008Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/85493 The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal Steyn, P.L. Buchan, L. Cloete, Thomas Eugene UCTD Detection of Acinetobacter Activated Sludge Biological Phosphorus Removal Thesis (DSc)--University of Pretoria, 1984. Biological phosphorus removal in activated sludge is not fully understood. Acinetobacter has been implicated as the principal phosphorus removing agent. A fluorescent antibody and Acridine Orange staining technique combined with mem brane filtration and epifluorescence microscopy was developed and applied for the in situ identification and enumeration of Acinetobacter and the total number of bacteria in activated sludge after chemical dispersion or sonication of activated sludge in a 0,5 % tripolyphosphate solution. Whereas it has been genera1ly assumed that all Acinetobacter cells were relatively large. the use of different membrane pore sizes combined with fluorescent antibody staining has shown that these cells can range from relatively very small to very large cells. This confirms the erroneous conclusions that can be made by considering morphological characteristics only for identification of bacteria using light microscopy. The Acinetobacter numbers in the different activated sludge zones differed significantly in time. The primary aerobic and secondary anoxic Acinctnhacter numbers differed significantly from the other zones when using membrane filters with a pore size of 0.45 μm suggesting that the size of these bacteria increased with higher metabolic activity in the primary aerobic zone resulting in carryover into the secondary anoxic zone. Although statistical analysis showed no correlation between Acinetobacter numbers and phosphorus removal in activated sludge, density gradient centrifugation of activated sludge revealed that Acinetobacter was associated with phosphorus in activated sludge. Using transmission electron microscopy, the cell volume of volutin containing cells and the volutin volume were determined. This confirmed that only large Acinetobacter cells contained phosphorus. Electron dispersive micro-analysis of X-rays (EDAX) confirmed that the volutin contained phosphorus. Using Acinetobacter numbers, volutin volumes, densities and phosphorus content it was determined that a maximum of 34% of the observed phosphorus removal in activated sludge could be removed by Acinetobacter as polyphosphate. Other organisms or mechanisms therefore, also had to be involved in the observed phosphorus removal. Microbiology and Plant Pathology DSc Unrestricted 2022-05-17T11:21:54Z 2022-05-17T11:21:54Z 21/7/2021 1984 Thesis * https://repository.up.ac.za/handle/2263/85493 en © 2020 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Detection of Acinetobacter Activated Sludge Biological Phosphorus Removal The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title | The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title_full | The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title_fullStr | The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title_full_unstemmed | The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title_short | The Detection of Acinetobacter In Activated Sludge and Its Possible Role in Biological Phosphorus Removal |
| title_sort | detection of acinetobacter in activated sludge and its possible role in biological phosphorus removal |
| topic | UCTD Detection of Acinetobacter Activated Sludge Biological Phosphorus Removal |
| url | https://repository.up.ac.za/handle/2263/85493 |