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Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa
Dissertation (MSc (Microbiology))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2022
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| _version_ | 1867613483533598720 |
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| access_status_str | Open Access |
| author2 | Coetzee, Martin Petrus Albertus |
| author_browse | Coetzee, Martin Petrus Albertus |
| author_facet | Coetzee, Martin Petrus Albertus |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/86195 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:52.135Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/86195 Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa Coetzee, Martin Petrus Albertus cindy.emofairy@gmail.com Steenkamp, Emma Theodora Ramokgano, Cindy Berchemia discolor Phylogenetic analyses Endophytic fungi Limpopo Province, South Africa UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2022. Previously, studies on Berchemia discolor trees focused on their pharmacological and nutritional properties. Despite the ecological, economical and pharmaceutical importance, little is known regarding the diseases affecting the species. One recently published paper dealing with fungi associated with B. discolor in Kenya reported 12 ascomycete species that cause dieback and cankers. Thus, the studies presented in this dissertation are the first that attempted to identify fungi associated with B. discolor in South Africa. A total of 29 species were tentatively identified from samples of B. discolor collected at different collection sites in the Limpopo Province of South Africa. These species were classified based on multi-gene DNA sequencing and the species belonged to the 17 genera within 10 families. out of the 29 species, eight species are potentially new to science. Botryosphaeriaceae species were the most notable and predominant in the natural ecosystems. Of the 29 species, 12 species overlapped on branches with dieback and asymptomatic branches, nine were isolated from branches with dieback, while 8 species were obtained from asymptomatic branches. No conclusive evidence could be found that the species obtained from the branches of B. discolor are the causal agents of the dieback disease, as Koch’s postulate was not applied in this study through pathogenicity trials. This study, however, should be seen as a foundational study as limited samples were collected from one province. The results from the chapters presented in this dissertation warrant further research in which the sampling areas and the number of sampled trees should be expanded in order to realise the full extent of the fungal species diversity on B. discolor and their potential impact on the health of these trees in South Africa. DSI-NRF Centre Of Excellence In Plant Health Biotechnology (CPHB) Microbiology and Plant Pathology MSc (Microbiology) Unrestricted 2022-07-14T14:09:17Z 2022-07-14T14:09:17Z 2022-09 2022 Dissertation Ramokgano, C 2022, Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed, 220627 https://repository.up.ac.za/handle/2263/86195 S2022 https://repository.up.ac.za/handle/2263/86195 10.25403/UPresearchdata.20158292 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Berchemia discolor Phylogenetic analyses Endophytic fungi Limpopo Province, South Africa UCTD Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title | Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title_full | Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title_fullStr | Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title_full_unstemmed | Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title_short | Diversity of endophytic fungi associated with branches of Berchemia discolor in the Limpopo Province of South Africa |
| title_sort | diversity of endophytic fungi associated with branches of berchemia discolor in the limpopo province of south africa |
| topic | Berchemia discolor Phylogenetic analyses Endophytic fungi Limpopo Province, South Africa UCTD |
| url | https://repository.up.ac.za/handle/2263/86195 |