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Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado
Dissertation (MSc (Microbiology))--University of Pretoria, 2022.
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| Format: | Thesis |
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University of Pretoria
2022
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| _version_ | 1867613547085692928 |
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| access_status_str | Open Access |
| author2 | Van den Berg, Noelani |
| author_browse | Van den Berg, Noelani |
| author_facet | Van den Berg, Noelani |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/86931 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:52.763Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/86931 Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado Van den Berg, Noelani Aaron.maringa@up.ac.za Swart, Velushka Maringa, Aaron UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2022. Phytophthora cinnamomi utilizes motile zoospores that chemotactically move towards a potential host to initiate infection. The release of adhesive material from encysting zoospores in P. cinnamomi confers a biological advantage to this pathogen and allows it to successfully attach. There is limited information on Phytophthora adhesion genes. In this study, putative adhesion genes were identified in the recently available P. cinnamomi GKB4 genome, these included six adhesins, five CBELs, and 23 Mucinlike proteins. Several adhesins, CBELs, and mucins were upregulated as early as 6 hours post inoculation (hpi) and late (120 hpi), indicating a role in the biotrophic and necrotrophic phases of infection. The adhesion of P. cinnamomi encysted zoospores on susceptible avocado (Ettinger) roots was examined through the use of scanning electron microscopy (SEM). SEM results showed that P. cinnamomi cysts can attach to the roots of avocado as early as 1 hpi and germinate predominantly at 3 and 6 hpi. Adhesive material was observed on the surface of cysts and germinating cysts. The expression profiles of three adhesion genes coding for adhesin-like (U6809), cellulose binding elicitor lectin (CBEL) (U6169), and mucin-like (U8949) proteins during avocado infection were generated from different stages of infection of Ettinger plants using quantitative Reverse Transcriptase PCR (qRT-PCR). The mucin gene was shown to be significantly upregulated at 3 and 6 hpi, indicating the potential role of this mucin in the early stages of avocado infection. P. cinnamomi appears to employ different adhesion genes during the different stages of avocado infection. This study provides a framework for further research aimed at understanding the role of adhesion in the P. cinnamomi-avocado interaction Microbiology and Plant Pathology MSc (Microbiology) Unrestricted 2022-08-23T14:14:38Z 2022-08-23T14:14:38Z 2022-09 2022-08-23 Dissertation * S2022 https://repository.up.ac.za/handle/2263/86931 © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title | Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title_full | Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title_fullStr | Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title_full_unstemmed | Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title_short | Investigating the adhesion of encysted zoospores of the oomycete Phytophthora cinnamomi during the early infection of avocado |
| title_sort | investigating the adhesion of encysted zoospores of the oomycete phytophthora cinnamomi during the early infection of avocado |
| topic | UCTD |
| url | https://repository.up.ac.za/handle/2263/86931 |