Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa
Dissertation (MSc (Tropical Animal Health))--University of Pretoria, 2020.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2022
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613705818079232 |
|---|---|
| access_status_str | Open Access |
| author2 | Van Heerden, Henriette |
| author_browse | Van Heerden, Henriette |
| author_facet | Van Heerden, Henriette |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Tropical Animal Health))--University of Pretoria, 2020. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/87901 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:23.989Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/87901 Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa Van Heerden, Henriette maggyhausiku@gmail.com Kolo, Francis B. Hausiku, Magrecia L. UCTD Brucellosis Abattoir Serology PCR Dissertation (MSc (Tropical Animal Health))--University of Pretoria, 2020. Background: Brucellosis is a contagious zoonotic bacterial disease of worldwide distribution and remains endemic in most low- and middle-income countries (LMICs), including South Africa. The current bovine brucellosis scheme in South Africa predominantly makes use of serological tests for diagnosis which lack 100% accuracy if the gold standard test (culturing) is not performed. Abattoirs can provide information on notifiable and zoonotic disease and can play a pivotal role in disease surveillance and monitoring. This study aimed at demonstrating the usefulness of abattoir surveillance for detection of Brucella spp. using serological, molecular and bacteriological methods. Methods: Serum and tissue samples (liver, spleen and lymph nodes) were collected from slaughter cattle at a local abattoir in Hammanskraal, Gauteng Province in September 2018. A total of 122 serum samples were screened for Brucella antibodies while molecular and bacteriological methods were used to detect Brucella spp. from tissue samples of serological positive animals. Results: The Rose Bengal test (RBT) revealed a seroprevalence of 22.1% (27/122, 95% CI = 15.1-30.5) and iELISA confirmed a seroprevalence of 9.0% (11/122, 95%CI = 4.6-15.6). Genus- specific 16S-23S rRNA interspacer region (ITS) - PCR detected Brucella DNA in 9 of the seropositive tissue samples of animals. From the 9 ITS-PCR positive animals, 44.4% (4/9) Brucella bacteria were isolated and confirmed to species level using the AMOS PCR assay. AMOS-PCR characterized the four Brucella isolates as B. abortus with one mixed culture consisting of B. abortus and B. melitensis in cattle. Conclusion: The findings conclude that abattoirs are facilities that can provide invaluable information on disease surveillance and that the gold standard procedure can be performed on collected tissue samples that yield in a more accurate diagnosis. Veterinary Tropical Diseases MSc (Tropical Animal Health) Unrestricted 2022-10-24T07:57:58Z 2022-10-24T07:57:58Z 2020 2020 Dissertation * A/S2021 https://repository.up.ac.za/handle/2263/87901 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Brucellosis Abattoir Serology PCR Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title | Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title_full | Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title_fullStr | Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title_full_unstemmed | Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title_short | Prevalence of brucellosis in cattle slaughtered in a local abattoir in Hammanskraal, South Africa |
| title_sort | prevalence of brucellosis in cattle slaughtered in a local abattoir in hammanskraal south africa |
| topic | UCTD Brucellosis Abattoir Serology PCR |
| url | https://repository.up.ac.za/handle/2263/87901 |