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Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence
Dissertation (MSc (Microbiology))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613577523757056 |
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| access_status_str | Open Access |
| author2 | Motaung, Thabiso Eric |
| author_browse | Motaung, Thabiso Eric |
| author_facet | Motaung, Thabiso Eric |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/88954 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:21.763Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/88954 Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence Motaung, Thabiso Eric chizne21@gmail.com Steenkamp, Emma Theodora Santana, Quentin Peremore, Chizne Biofilms Fusarium verticillioides Extracellular vesicles Extracellular polymeric substance Extracellular DNA UCTD Dissertation (MSc (Microbiology))--University of Pretoria, 2022. Extracellular vesicles (EVs) have substantial functional consequences in fungi because they internalize bioactive molecules (e.g., proteins, nucleic acids, and secondary metabolites) from their source cells. Internalized contents can be released into the extracellular environment, where they can either persist for long periods of time because they are protected by the vesicle membrane from potentially harmful agents (e.g., degradative enzymes), ensuring their potency, or they can be readily absorbed by recipient cells, causing a variety of physiological effects. In fungi such as Candida albicans, EVs enhance virulence factor production such as biofilms, a matrixed and architecturally complex microbial community typically attached to a surface and embedded in a gelatinous matrix composed of extracellular polymeric substances (EPS). As EVs are also produced by biofilms, which are a key virulence determinant in nearly all microbial pathogens, a thorough understanding of their role in biofilm formation is key. During this study, it was first thoroughly characterized biofilm formation in the important disease-causing agent in the maize crop, Fusarium verticillioides. Then, EVs were recovered and characterized from free-living (planktonic) cells and biofilms of Fusarium verticillioides, offering fresh information on how key signals (e.g., nutrients, enzymes, genes, RNA, DNA, metabolites, and so on) are exchanged to contribute to biofilm development and structural integrity. Given that both EV and biofilm biology in phytopathogens are poorly understood, these findings will greatly contribute to this knowledge gap and serve as a reference point for further research into biofilms as a poorly represented virulence determinant in fungal phytopathogens. NRF Biochemistry, Genetics and Microbiology (BGM) MSc (Microbiology) Unrestricted 2023-01-25T09:29:46Z 2023-01-25T09:29:46Z 2023-04 2022 Dissertation * A2023 https://repository.up.ac.za/handle/2263/88954 10.25403/UPresearchdata.21922233 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Biofilms Fusarium verticillioides Extracellular vesicles Extracellular polymeric substance Extracellular DNA UCTD Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title | Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title_full | Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title_fullStr | Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title_full_unstemmed | Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title_short | Biofilms and extracellular vesicles of Fusarium verticillioides and their implications for virulence |
| title_sort | biofilms and extracellular vesicles of fusarium verticillioides and their implications for virulence |
| topic | Biofilms Fusarium verticillioides Extracellular vesicles Extracellular polymeric substance Extracellular DNA UCTD |
| url | https://repository.up.ac.za/handle/2263/88954 |