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Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction
Dissertation (MSc Agric Animal Science (Animal Nutrition))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613614036221952 |
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| access_status_str | Open Access |
| author2 | Hassen, Abubeker |
| author_browse | Hassen, Abubeker |
| author_facet | Hassen, Abubeker |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc Agric Animal Science (Animal Nutrition))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/89147 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:56.612Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/89147 Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction Hassen, Abubeker bakareimranab@gmail.com Van Marle-Koster, Este Morgavi, Diego Lawal, Imrana-Bakare Methane reduction Microbes Rumen Tannin Metagenome UCTD Dissertation (MSc Agric Animal Science (Animal Nutrition))--University of Pretoria, 2023. Methane (CH4) is one of the primary gases that contribute to global warming. It is a by-product of enteric fermentation of ruminant animals, which is produced by microbes (methanogens that belong to domain Archaea) in the rumen. The emission of methane from the ruminant can be reduced to varying degrees through the manipulation of the rumen microbiome by various dietary interventions. It has been well established that diet affects the microbial community structure and composition. Tannins have been shown to directly or indirectly inhibit methanogenesis, thereby reducing methane. However, the effect of encapsulated tannins on the microbial diversity in the rumen has not been fully understood. In this study, 24 rumen samples were analysed from a study where Eragrostis curvula based diet was fed to South Africa Mutton Merino sheep, and was supplemented with tannin crude or encapsulated tannin. DNA were extracted and sequenced using shotgun metagenomic and analysed using MG-RAST. Out of the 28 bacteria phyla identified by shotgun sequencing, Bacteroidetes (72%) and Firmicutes (21%) were the dominant phyla. A total of 500 bacterial genera were recorded, where Prevotella, Bacteroides, Eubacterium and Clostridium had the highest abundance. Forty-one archaeal genera were identified with Methanobrevibacter having the highest abundance. However, the total bacteria and total methanogen did not significantly differ between the tannin and non-tannin treatments. This shows that tannin in its crude or encapsulated form did not have any effect on the methane producing microbes and the overall microbial community. Animal and Wildlife Sciences MSc Agric Animal Science (Animal Nutrition) Unrestricted 2023-02-03T13:37:46Z 2023-02-03T13:37:46Z 2023-04-03 2023-01 Dissertation * https://repository.up.ac.za/handle/2263/89147 10.25403/UPresearchdata.21959618 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Methane reduction Microbes Rumen Tannin Metagenome UCTD Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title | Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title_full | Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title_fullStr | Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title_full_unstemmed | Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title_short | Changes in microbial population in the rumen of sheep fed Acacia mearnsii tannin extract for methane reduction |
| title_sort | changes in microbial population in the rumen of sheep fed acacia mearnsii tannin extract for methane reduction |
| topic | Methane reduction Microbes Rumen Tannin Metagenome UCTD |
| url | https://repository.up.ac.za/handle/2263/89147 |