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Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium
Dissertation (MEng (Chemical Engineering))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613628112306176 |
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| access_status_str | Open Access |
| author2 | Brink, Hendrik Gideon |
| author_browse | Brink, Hendrik Gideon |
| author_facet | Brink, Hendrik Gideon |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MEng (Chemical Engineering))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/89249 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:09.918Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/89249 Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium Brink, Hendrik Gideon u15037780@tuks.co.za Chirwa, Evans M.N. Neveling, Olga UCTD Klebsiella pneumoniae Biosorption Lead Removal Paraclostridium bifermentans Precipitation Engineering, built environment and information technology theses SDG-03 Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Engineering, built environment and information technology theses SDG-14 Dissertation (MEng (Chemical Engineering))--University of Pretoria, 2022. The study focused on determining the microbial precipitation abilities of two bacterial strains, Paraclostridium bifermentans and Klebsiella pneumoniae isolated from an industrially obtained microbial consortium. Previous research has demonstrated the effectiveness of the consortium in the bioprecipitation of Pb(II). The bioremediation of Pb(II) provides an alternative and less costly method for lead removal from solution. A proof of concept was determined in a long duration study over 100 h wherein the bioprecipitation abilities of the strains were determined. It was concluded that approximately 84 % and 100 % of Pb(II) was removed from solution in experiments containing 80 mg/L initial Pb(II) concentration over 100 h, with P. bifermentans and K. pneumoniae respectively. The mechanisms of precipitation were further investigated with a short-term study, since it was observed that precipitation occurred in under 18 h in the long duration study. This shorter study was conducted over 30 h with nine sampling intervals and indicated removal percentages of approximately 86 % and 91 % for samples containing 80 mg/L initial Pb(II) concentration after 30 h for P. bifermentans and K. pneumoniae respectively. The precipitate identity was determined to be PbS and Pb(0) for samples containing P. bifermentans while samples containing K. pneumoniae contained precipitates of PbO and either PbCl or Pb3(PO4)2. An investigation into the extracellular and intracellular Pb(II) concentration led to the observation that a rapid detoxification mechanism such as biosorption is present in the microbes within the first 6 h of the experiment. These mentioned factors provide a greater understanding of the mechanisms utilised by the bacteria in the bioprecipitation and adsorption of Pb(II), and can be used as a step towards applying the process on an industrial scale. National Research Fund (120321, 145848, 121891 and 128088). mi2026 Chemical Engineering MEng (Chemical Engineering) Unrestricted SDG-03: Good health and well-being SDG-06: Clean water and sanitation SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production SDG-14: Life below water 2023-02-07T12:51:14Z 2023-02-07T12:51:14Z 2023 2022 Dissertation * A2023 https://repository.up.ac.za/handle/2263/89249 https://doi.org/10.25403/UPresearchdata.22010558 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Klebsiella pneumoniae Biosorption Lead Removal Paraclostridium bifermentans Precipitation Engineering, built environment and information technology theses SDG-03 Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Engineering, built environment and information technology theses SDG-14 Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title | Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title_full | Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title_fullStr | Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title_full_unstemmed | Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title_short | Microbial precipitation of Pb(II) with wild strains of Klebsiella Pneumoniae and Paraclostridium Bifermentans isolated from an industrially obtained microbial consortium |
| title_sort | microbial precipitation of pb ii with wild strains of klebsiella pneumoniae and paraclostridium bifermentans isolated from an industrially obtained microbial consortium |
| topic | UCTD Klebsiella pneumoniae Biosorption Lead Removal Paraclostridium bifermentans Precipitation Engineering, built environment and information technology theses SDG-03 Engineering, built environment and information technology theses SDG-06 Engineering, built environment and information technology theses SDG-09 Engineering, built environment and information technology theses SDG-12 Engineering, built environment and information technology theses SDG-14 |
| url | https://repository.up.ac.za/handle/2263/89249 https://doi.org/10.25403/UPresearchdata.22010558 |