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In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants
Dissertation (MSc (Genetics))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613706691543040 |
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| access_status_str | Open Access |
| author2 | Moela, Pontsho |
| author_browse | Moela, Pontsho |
| author_facet | Moela, Pontsho |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Genetics))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/89435 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:24.731Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/89435 In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants Moela, Pontsho angelabona95@gmail.com October, Natasha Bona, Angela UCTD Cervical cancer Anti-cancer compound Plant compound Compound combination Drug discovery Dissertation (MSc (Genetics))--University of Pretoria, 2023. Cervical cancer is a public female health burden, especially in Africa, and is mainly caused by infection with HPV in which unvaccinated cases allow the development of malignancy and ultimately angiogenesis and metastasis. Apoptosis, which is often evaded in cancer, is a popular targeted mechanism of current and potential anti-cancer drugs. However, cytotoxic cervical cancer therapies, such as platinum-based chemo- and radiotherapy, also elicits non-selective and systemic toxicity and temporarily subdues advanced cancers into remission with unexpected relapse. In addition, treatments utilizing an apoptotic anti-cancer approach induce necrosis concomitantly which result in inflammatory side-effects. This necessitates the screening of alternative treatment regimens, such as plant secondary metabolites and their various combinations for discovery of new anti-cancer compounds or unprecedented anti-cancer potentials of old compounds. Therefore, compounds isolated from four Cameroonian plants, namely Cassia arereh, Distemonanthus benthamianus, Echinops gracilis and Rhabdophyllum affine, were screened for their individual cytotoxicities against cervical cancer cells. Compounds that showed sufficient anti-cancer and cancer-selective potentials were then further studied in combination. Initial screening revealed a terpenoid and flavonoid isolated from C. arereh (CAE21) and E. gracilis (EGF25), respectively. CAE21 and EGF25 induced strong apoptotic responses especially in combination with no necrotic response. In addition, CAE21 showed optimal oral bioavailability in silico, although EGF25 did not show the same drug-likability. CAE21 and EGF25 also demonstrated molecular bioactivities of 0.18 and 0.14 as GPCR and nuclear receptor ligands, respectively. Despite the demonstrated anti-cancer potential of CAE21 and EGF25, reproducible effects in more biological cancer models and further elucidation of apoptotic and molecular mechanisms are needed. National Research Foundation (NRF), Grant UID 113980. South African Medical Research Council (A1A979). Genetics MSc (Genetics) Unrestricted 2023-02-13T10:01:15Z 2023-02-13T10:01:15Z 2023-04 2023 Dissertation Bona, A 2023, In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants, MSc Dissertation, University of Pretoria, Pretoria, viewed yymmdd https://repository.up.ac.za/handle/2263/89435 A2023 https://repository.up.ac.za/handle/2263/89435 DOI: 10.25403/UPresearchdata.22068350 https://doi.org/10.25403/UPresearchdata.22068350 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Cervical cancer Anti-cancer compound Plant compound Compound combination Drug discovery In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title | In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title_full | In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title_fullStr | In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title_full_unstemmed | In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title_short | In vitro screening in cervical cancer cells of anti-cancer compounds derived from Cameroonian plants |
| title_sort | in vitro screening in cervical cancer cells of anti cancer compounds derived from cameroonian plants |
| topic | UCTD Cervical cancer Anti-cancer compound Plant compound Compound combination Drug discovery |
| url | https://repository.up.ac.za/handle/2263/89435 https://doi.org/10.25403/UPresearchdata.22068350 |