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The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood
Dissertaion (MSc (Human Physiology))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613624761057280 |
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| access_status_str | Open Access |
| author2 | Bester, Janette |
| author_browse | Bester, Janette |
| author_facet | Bester, Janette |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertaion (MSc (Human Physiology))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/89694 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:06.697Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/89694 The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood Bester, Janette u16107803@tuks.co.za Strydom, Morne Chamboko, Tanyaradzwa Dellah UCTD Clot Fibrin fibres Platelets Red blood cells Venom Ultrastructure Dissertaion (MSc (Human Physiology))--University of Pretoria, 2023. Snake bites are of a major global concern that may affect any individual in all age groups, gender, geographical location and economic status. Previous research has identified the effects of venomous snake bites of various species and families and the correlation with proteins found in snake venom. There is, however, limited research on the effects of the Naja mossambica (Mozambique spitting cobra) venom on the coagulation system, particularly the haematological system and blood rheology. In order to better understand the effects of Naja mossambica venom on the coagulation system, this research aimed to study the ex vivo physiological effects of Naja mossambica venom, of concentration 0.025 ng/μL, on human blood cells and the viscoelastic properties of the coagulation system. The methods used include light microscopy (LM) and scanning electron microscopy (SEM), which provided morphological data on red blood cells (RBCs), platelets, fibrin fibres and clot structure, particularly with the SEM. In addition, Thromboelastography® (TEG®) was performed to study clot kinetics and clot formation and the Global thrombosis test® (GTT®) was performed to study platelet activity in relation to thrombin generation and thrombolysis. From the results, it was established that there was an increase in deformed RBCs, increased platelet activity, and fibrin fibres appeared loosely packed. Also, a significant difference in the clot kinetics was noted, suggesting changed fibrin mechanics. Therefore, these results suggest hypo-coagulability in individuals exposed to Naja mossambica snake venom. The hypo-coagulable state in the victims might not be to an extent of bleeding events, however, this might have implications in blood flow and wound healing. This information can also be used in future studies to improve diagnostics or management strategies for snakebite victims, and to contribute to the haemotoxic profile of venomous snakes. National Research Foundation (NRF) Postgraduate Scholarship Physiology MSc (Human Physiology) Unrestricted 2023-02-20T09:37:06Z 2023-02-20T09:37:06Z 2023-04 2023 Dissertation * A2023 https://repository.up.ac.za/handle/2263/89694 DOI: 10.25403/UPresearchdata.22117316 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Clot Fibrin fibres Platelets Red blood cells Venom Ultrastructure The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title | The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title_full | The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title_fullStr | The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title_full_unstemmed | The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title_short | The ex vivo effects of Naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| title_sort | ex vivo effects of naja mossambica venom on the ultrastructure and viscoelastic properties of human blood |
| topic | UCTD Clot Fibrin fibres Platelets Red blood cells Venom Ultrastructure |
| url | https://repository.up.ac.za/handle/2263/89694 |