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Aggressiveness of diverse African isolates of Cercospora zeina on maize
Dissertation (MSc Agric (Plant pathology))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613519385460736 |
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| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc Agric (Plant pathology))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/90182 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:26.341Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/90182 Aggressiveness of diverse African isolates of Cercospora zeina on maize Berger, David Kenneth u14237459@tuks.co.za Nsibo, David L. Mokgobu, Tumisang UCTD Cercospora zeina Koch’s postulate Grey leaf spot Epidemiology Quantitative traits Aggressiveness Koch’s postulate Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-12 Dissertation (MSc Agric (Plant pathology))--University of Pretoria, 2022. Cercospora zeina the causal agent of grey leaf spot of maize causes up to 67% yield losses when susceptible maize cultivars are planted. Variations in the levels of aggressiveness of C. zeina isolates have been reported previously. However, none of the previous studies links the variation in their levels of aggressiveness to the genetic diversity. This study links the genetic diversity within and between African populations of C. zeina isolates to differences in aggressiveness. In this study, the variation in the aggressiveness of five C. zeina isolates (CEDV05074, CMW25467, ZWRRS263, UGKPC38, and KEKER468) was assessed using six quantitative traits namely, incubation period, infection efficiency, lesion size, disease severity index (DSI), area under disease progression curve (AUDPC) and sporulation capacity. Our findings reveal that the genetic diversity previously observed in the C. zeina isolates influences their level of aggressiveness. From this study, we noted the need to standardize the protocol for assessing the infection efficiency and the timing for measuring lesion size in the maize-C. zeina pathosystem. Our findings also proved that hybrid-3 was the most susceptible hybrid and therefore will be used for future aggressiveness assays. Lastly, the identity of the five C. zeina isolates was confirmed using four molecular tools namely, CTB7 gene region, mating type genes, internal transcribed spacer (ITS) gene region, and microsatellite markers. Overall, this study is a step towards mechanisms involved in the pathogenicity of C. zeina. Furthermore, using isolates with high aggressiveness is key for maximizing selection gain when using artificial inoculation to assess maize plant resistance to GLS disease. South African National Seed Organization (SANSOR). National Research Foundation (NRF) grant holder linked bursary. bs2026 Plant Science MSc Agric (Plant pathology) Unrestricted SDG-02: Zero hunger SDG-09: Industry, innovation and infrastructure SDG-12: Responsible consumption and production 2023-03-23T06:48:20Z 2023-03-23T06:48:20Z 2023-09 2022 Dissertation * S2023 http://hdl.handle.net/2263/90182 https://doi.org/10.25403/UPresearchdata.22219069.v1 en © 2022 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Cercospora zeina Koch’s postulate Grey leaf spot Epidemiology Quantitative traits Aggressiveness Koch’s postulate Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-12 Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title | Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title_full | Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title_fullStr | Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title_full_unstemmed | Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title_short | Aggressiveness of diverse African isolates of Cercospora zeina on maize |
| title_sort | aggressiveness of diverse african isolates of cercospora zeina on maize |
| topic | UCTD Cercospora zeina Koch’s postulate Grey leaf spot Epidemiology Quantitative traits Aggressiveness Koch’s postulate Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-12 |
| url | http://hdl.handle.net/2263/90182 https://doi.org/10.25403/UPresearchdata.22219069.v1 |