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The influence of temperature on bacterial communities in arid soil microbiomes
Thesis (MSc (Microbiology))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613543406239744 |
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| access_status_str | Open Access |
| author2 | Makhalanyane, Thulani Peter |
| author_browse | Makhalanyane, Thulani Peter |
| author_facet | Makhalanyane, Thulani Peter |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (MSc (Microbiology))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/90688 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:49.221Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/90688 The influence of temperature on bacterial communities in arid soil microbiomes Makhalanyane, Thulani Peter mablebaluka@gmail.com Dr Koopman, Jessica E. Baluka, Mable Rosula UCTD 16S rRNA gene Bacterial diversity Ddrylands Soil physicochemical variables Temperature Thesis (MSc (Microbiology))--University of Pretoria, 2023. Despite their profound effects, we lack comprehensive mechanistic insights regarding the effects of temperature increases on dryland plant-associated microbiomes. This knowledge deficit is especially true for prominent plants in understudied dryland soils from Africa. To establish a baseline for predictive studies, we constructed soil microcosms to test the effects of environmental perturbations on microbial communities. Samples associated with prominent arid zone tree Acacia erioloba were collected. To study the effects of temperature, we incubated soils at 30°C and 40°C, respectively, for forty days. We used 16S rRNA gene amplicon sequencing and soil physicochemical parameters to determine bacterial diversity and the drivers of microbial community structure. The results suggest that some soil physicochemical variables may be more substantial drivers of microbial communities, masking the effects of temperature increases. In general, bacterial communities were dominated by ubiquitous taxa including Actinobacteria (39.1%), Firmicutes (36.1%), Proteobacteria (9.9%), Acidobacteria (4.6%), Chloroflexi (4.5%). Microhabitats differences appear to explain bacterial community composition, with higher relative abundances of Firmicutes in canopy soils and Actinobacteria and Chloroflexi in open soils. Distance-based redundancy analysis suggests that iron was a major determinant of bacterial diversity in canopy soils. However, other factors including copper and pH appear to be major drivers explaining bacterial community structure in open soils. Taken together, the data suggest that physicochemical variables in microhabitats may buffer the effects of short-term temperature increases on soil microbes in arid soils. The research was funded by the Foundational Biodiversity Program of the National Research Foundation (NRF) (UID number: 114412). Microbiology and Plant Pathology MSc (Microbiology) Unrestricted 2023-05-15T12:21:31Z 2023-05-15T12:21:31Z 2023-09 2023 Thesis * S2023 http://hdl.handle.net/2263/90688 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD 16S rRNA gene Bacterial diversity Ddrylands Soil physicochemical variables Temperature The influence of temperature on bacterial communities in arid soil microbiomes |
| title | The influence of temperature on bacterial communities in arid soil microbiomes |
| title_full | The influence of temperature on bacterial communities in arid soil microbiomes |
| title_fullStr | The influence of temperature on bacterial communities in arid soil microbiomes |
| title_full_unstemmed | The influence of temperature on bacterial communities in arid soil microbiomes |
| title_short | The influence of temperature on bacterial communities in arid soil microbiomes |
| title_sort | influence of temperature on bacterial communities in arid soil microbiomes |
| topic | UCTD 16S rRNA gene Bacterial diversity Ddrylands Soil physicochemical variables Temperature |
| url | http://hdl.handle.net/2263/90688 |