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Genome‐wide analysis of fitness data in Ceratocystis albifundus
Dissertation (MSc (Microbiology))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613592859181056 |
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| access_status_str | Open Access |
| author2 | Van der Nest, Magrieta Aletta |
| author_browse | Van der Nest, Magrieta Aletta |
| author_facet | Van der Nest, Magrieta Aletta |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/91153 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:35.948Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/91153 Genome‐wide analysis of fitness data in Ceratocystis albifundus Van der Nest, Magrieta Aletta vinoli.damki@up.ac.za Steenkamp, Emma Theodora Wingfield, Brenda D. De Vos, Lieschen Mchunu, Mchunu Danki, Vinolia Nomhle UCTD Fitness Ceratocystis albifundus Host-specificity Fungi Southern Africa Dissertation (MSc (Microbiology))--University of Pretoria, 2023. Ceratocystis albifundus is an emerging pathogen of non-native Acacia mearnsii in Southern Africa. Although the fungus generally does not cause disease symptoms on native hosts, disease symptoms have been recently observed on commercial Protea cynaroides. Since laccases, an oxidase enzyme, have been implicated to influence plant-pathogen interactions, this study aimed to use a genome-wide association study (GWAS) approach to identify genes and pathways associated with oxidase activity across a South African C. albifundus population originating from a wide geographic and host range. These individuals were genotyped using low-coverage genome sequencing technologies and laccase activity was determined for each isolate. This study demonstrates that oxidative response represents a quantitative trait in this species and is associated with multiple genomic regions. This implies that oxidative activity may influence plant-pathogen interactions. Correlation between the single nucleotide polymorphism data for the isolates and their corresponding phenotype information allowed for the identification of a collection of genomic regions that were significantly associated with oxidase activity. For example, one of the regions contained a gene that codes for a protein in the multicopper oxidase superfamily, of which laccase is a member. However, further research experiments are required to validate computational study, with the ultimate goal, of improving our knowledge regarding pathogenesis in this economically important fungus. Forestry, Agricultural, and Biotechnology Institute (FABI) NRF Microbiology and Plant Pathology MSc(Microbiology) Unrestricted 2023-06-20T09:04:11Z 2023-06-20T09:04:11Z 2023 2023 Dissertation * S2023 http://hdl.handle.net/2263/91153 DOI: https://doi.org/10.25403/UPresearchdata.23544654.v1 https://doi.org/10.25403/UPresearchdata.23544654 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Fitness Ceratocystis albifundus Host-specificity Fungi Southern Africa Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title | Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title_full | Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title_fullStr | Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title_full_unstemmed | Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title_short | Genome‐wide analysis of fitness data in Ceratocystis albifundus |
| title_sort | genome wide analysis of fitness data in ceratocystis albifundus |
| topic | UCTD Fitness Ceratocystis albifundus Host-specificity Fungi Southern Africa |
| url | http://hdl.handle.net/2263/91153 https://doi.org/10.25403/UPresearchdata.23544654 |