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Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies
Dissertation (MSc (Microbiology))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613617182998528 |
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| access_status_str | Open Access |
| author2 | Theron, Jacques |
| author_browse | Theron, Jacques |
| author_facet | Theron, Jacques |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/91745 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:59.552Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/91745 Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies Theron, Jacques ginaroeb@gmail.com Roebuck, Gina African horse sickness virus viruses UCTD Orbivirus Protein phoshorylation Virus inlcusion bodies Nonstructural protein NS2 Dissertation (MSc (Microbiology))--University of Pretoria, 2023. African horse sickness virus (AHSV), a member of the Orbivirus genus (family Sedoreoviridae, order Reovirales), encodes seven structural proteins and four non-structural proteins. The non-structural protein NS2 is the only virus-specified phosphoprotein and is the main component of the characteristic virus inclusion bodies (VIBs) observed in the cytoplasm of orbivirus-infected cells. Although VIBs are considered the sites at which virus replication and assembly occurs, the factors that govern their formation is still poorly understood in the case of AHSV. By making use of in vitro phosphorylation assays, kinase inhibitor studies, phosphoprotein staining assays and immunofluorescent confocal microscopy, wild-type NS2 and NS2 mutant proteins were characterized. Based on the results, it can be concluded that NS2 of AHSV-4 is phosphorylated by Casein kinase 2 at serine residues 256 and 258, and that phosphorylation of the NS2 protein is required for the formation of intracellular virus inclusion body-like structures. Poliomyelitis Research Foundation (Grant 18/89) and the University of Pretoria (UP Postgraduate Masters Research Bursary) Microbiology and Plant Pathology MCs (Microbiology) Unrestricted 2023-08-01T09:25:27Z 2023-08-01T09:25:27Z 2023-09-20 2023 Thesis * S2023 http://hdl.handle.net/2263/91745 https://figshare.com/s/5044ec74db75c1918c08 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | African horse sickness virus viruses UCTD Orbivirus Protein phoshorylation Virus inlcusion bodies Nonstructural protein NS2 Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title | Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title_full | Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title_fullStr | Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title_full_unstemmed | Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title_short | Importance of African horse sickness virus NS2 protein phosphorylation for the formation of viral inclusion bodies |
| title_sort | importance of african horse sickness virus ns2 protein phosphorylation for the formation of viral inclusion bodies |
| topic | African horse sickness virus viruses UCTD Orbivirus Protein phoshorylation Virus inlcusion bodies Nonstructural protein NS2 |
| url | http://hdl.handle.net/2263/91745 https://figshare.com/s/5044ec74db75c1918c08 |