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Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa
Dissertation (MSc (Medical Virology))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613692384772096 |
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| access_status_str | Open Access |
| author2 | Venter, Marietjie |
| author_browse | Venter, Marietjie |
| author_facet | Venter, Marietjie |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Virology))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/91954 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:10.900Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/91954 Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa Venter, Marietjie clourens101@gmail.com Mendes, Adriano Lourens, Carla UCTD Surveillance of arboviruses Neurological diseases Enzyme-linked immunosorbent assay (ELISA) Serological assays Antibodies Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-08 SDG-08: Decent work and economic growth Health sciences theses SDG-17 SDG-17: Partnerships for the goals Health sciences theses SDG-15 SDG-15: Life on land Dissertation (MSc (Medical Virology))--University of Pretoria, 2023. The burden of WNV and association with neurological disease was identified through syndromic surveillance in animals and humans using available serological tools. WSLB virus was identified in two samples (an equine and a bird) in South Africa for the first time since 2008. Additionally, ELISA’s were developed testing for IgG and IgM antibodies in equines, humans, cattle, and a species-independent assay for WNV and WSLB using the NS1 antigen, which differs from the commercial ELISA’s available that targets the E protein. The human WNV IgM and IgG ELISA showed very promising results, while the equine WNV ELISA’s had quite high background and this could be due to the different target antigen or the use of a baculovirus expressed protein with commercial antibodies. The cattle WNV IgM ELISA also showed good results and can possibly be implemented in the lab. The WSLB ELISA’s were only developed and optimized with rabbit sera, since a clinical sample lacked neutralizing antibodies for use in the assay. Unfortunately, the baculovirus expression of the NS1 protein was unsuccessful. LEAP-Agri, LEARN (grant number:115574). ANDEMIA project (grant number: 01KA1606). G7 Global Health Fund (grant number: ZMVI1-2517GHP703). Poliomyelitis Research Foundation. University of Pretoria Postgraduate bursary. em2025 Medical Virology MSc (Medical Virology) Unrestricted SDG-03: Good health and well-being SDG-08: Decent work and economic growth SDG-17: Partnerships for the goals SDG-15: Life on land 2023-08-17T06:54:32Z 2023-08-17T06:54:32Z 2023-09-08 2023 Dissertation * S2023 http://hdl.handle.net/2263/91954 DOI: https://doi.org/10.25403/UPresearchdata.23968620.v1 10.25403/UPresearchdata.23968620 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Surveillance of arboviruses Neurological diseases Enzyme-linked immunosorbent assay (ELISA) Serological assays Antibodies Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-08 SDG-08: Decent work and economic growth Health sciences theses SDG-17 SDG-17: Partnerships for the goals Health sciences theses SDG-15 SDG-15: Life on land Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title | Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title_full | Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title_fullStr | Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title_full_unstemmed | Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title_short | Establishment of serological assays to investigate the incidence of West Nile and Wesselsbron flavivirus infections in Africa |
| title_sort | establishment of serological assays to investigate the incidence of west nile and wesselsbron flavivirus infections in africa |
| topic | UCTD Surveillance of arboviruses Neurological diseases Enzyme-linked immunosorbent assay (ELISA) Serological assays Antibodies Health sciences theses SDG-03 SDG-03: Good health and well-being Health sciences theses SDG-08 SDG-08: Decent work and economic growth Health sciences theses SDG-17 SDG-17: Partnerships for the goals Health sciences theses SDG-15 SDG-15: Life on land |
| url | http://hdl.handle.net/2263/91954 |