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Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum)
Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2023
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| _version_ | 1867613704344829952 |
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| access_status_str | Open Access |
| author2 | Holm, Dietmar Erik |
| author_browse | Holm, Dietmar Erik |
| author_facet | Holm, Dietmar Erik |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/92186 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:22.637Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/92186 Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) Holm, Dietmar Erik Liamemarais@gmail.com Smuts, M.P. (Mario) Durrant, Barbara S. Marais, Liamé UCTD Assisted reproductive technology Ovum pick-up Southern white rhinoceros Temperature control Oocyte transport Dissertation (MSc (Veterinary Science))--University of Pretoria, 2022. Assisted reproductive technologies need to be developed to assist in the battle against the extinction of endangered and threatened species. Temperature control during oocyte recovery and transportation is essential for ensuring in vitro embryo development. In this study, we examined the temperature pathway of oocyte aspiration fluid during ovum pick-up (OPU) in the southern white rhino (Ceratotherium simum simum) in South Africa, and its role in oocyte maturation success. The large aspiration instrument used was made from PVC plastic. As a poor heat conductor, it needed to be pre-heated for several hours before the OPU procedure commenced to avoid cooling of the oocytes. Fewer temperature fluctuations were recorded when oocytes were transported in a portable transport heating/cooling incubator than in an Equitainer. A greater number of oocytes were harvested per aspirated follicle during the suggested predominant breeding season (November – April), after adjusting for the duration of the OPU procedure. Oocytes harvested from rhino cows given a warm-water enema prior to the OPU procedure were 2.31 times more likely to mature in vitro than those harvested from cows receiving a cold-water enema, after adjusting for the individual effect of the rhino and the negative effect of follicular wave stimulation on the oocyte maturation success. This study indicates that to ensure the successful maturation of oocytes in southern white rhino, oocyte hypothermia during aspiration and transportation is a major concern. The results and conclusions of this study may inform future research with respect to temperature control of oocytes during recovery and transportation for other endangered wildlife species. Production Animal Studies MSc (Veterinary Science) Unrestricted 2023-09-04T13:12:58Z 2023-09-04T13:12:58Z 2023-04 2022-06 Dissertation * A2023 http://hdl.handle.net/2263/92186 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Assisted reproductive technology Ovum pick-up Southern white rhinoceros Temperature control Oocyte transport Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title | Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title_full | Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title_fullStr | Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title_full_unstemmed | Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title_short | Monitoring and controlling aspiration fluid temperature during ovum pick-up in southern white rhinoceros (Ceratotherium simum simum) |
| title_sort | monitoring and controlling aspiration fluid temperature during ovum pick up in southern white rhinoceros ceratotherium simum simum |
| topic | UCTD Assisted reproductive technology Ovum pick-up Southern white rhinoceros Temperature control Oocyte transport |
| url | http://hdl.handle.net/2263/92186 |