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Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry
Dissertation (MSc (Environmental Technology)) -- University of Pretoria, 2023.
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| Format: | Thesis |
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University of Pretoria
2023
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| _version_ | 1867613551974154240 |
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| access_status_str | Open Access |
| author2 | Brink, Hendrik Gideon |
| author_browse | Brink, Hendrik Gideon |
| author_facet | Brink, Hendrik Gideon |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Environmental Technology)) -- University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/92775 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:57.427Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/92775 Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry Brink, Hendrik Gideon u21798894@tuks.co.za Chirwa, Evans M.N. Kpai, Yawo Patrick UCTD Adsorption Consortium K. pneumonia P. bifermentans Sewage sludge Bran-based filler bacteria Salt-and-starch filler bacteria Dissertation (MSc (Environmental Technology)) -- University of Pretoria, 2023. The current study aimed at investigating the bioremediation removal effectiveness of Pb(II) by metabolically inhibited microbial cultures: a) Waste activated sewage sludge obtained from a local wastewater treatment plant (SS), commercially sourced industrial bioremediation microbes b) bran-based filler with bacteria (BB), and c) salt-and-starch based filler with bacteria (S&S), d) an industrially obtained Pb(II) remediating consortium (Cons), and purified strains of e) Paraclostridium bifermentans (PB), and f) Klebsiella pneumoniae (KP) isolated from the consortium. The study’s focus was specifically targeted towards operational analysis. This study demonstrated that the metabolically inactive SS, BB, S&S, Cons, PB, and KP bacteria adsorbed 55.4 mg/g, 54.6 mg/g, 50.6 mg/g, 54.4 mg/g, 27.4 mg/g, and 23.1 mg/g of Pb(II) within 3 h, respectively. In addition, maximum adsorption capacities of 141.2 mg/g, 208.5 mg/g, 193.8 mg/g, 220.4 mg/g, 153.2 mg/g, and 217.7 mg/g were measured respectively. FTIR spectroscopy supported the chemisorption of Pb(II) onto functional groups as being responsible for this removal. Two-phase pseudo-first-order kinetics fits best described the adsorption kinetics of the adsorbents which might be as a result of the separation of fast and slow adsorption rates into separate compartments, thereby allowing for better representation of a heterogeneous surface. The Crank mass transfer model shows that external mass transfer is the main mechanism of Pb(II) removal due to the high molecular diffusivity of Pb(II) as compared to the effective diffusion coefficients of the metabolically inhibited adsorbents. The equilibrium isotherm was well described by two-surface Langmuir equilibrium isotherm model likely due to different adsorption sites with different adsorption energies which allows a comparably better description of the adsorption. The morphology of the adsorbents showed that the surface of the metabolically inhibited adsorbents was rough, coarse, with observable pores, and irregular iii crevices. The results from the EDS analyses indicated the presence of Pb on the surface of the metabolically inhibited adsorbents confirming the adsorbents were able to remove Pb(II) from the aqueous systems. Recovery of Pb(II) from the biosorbents were further tested and showed 72.4 %, 68.6 %, 69.7 %, 69.6 %, 61.0 %, and 72.4 % for the SS, BB, S&S, Cons, PB, and KP bacteria, respectively. The results demonstrate the remarkable potential of these low cost, self- generating biosorbents for the treatment of Pb(II) contaminated aqueous streams. Mastercard Foundation Scholarship, University of Pretoria National Research Fund, South Africa. Chemical Engineering MSc Unrestricted 2023-10-09T09:50:27Z 2023-10-09T09:50:27Z 2024-05 2023 Dissertation * http://hdl.handle.net/2263/92775 DOI: https://doi.org/10.25403/UPresearchdata.24276937.v1 10.25403/UPresearchdata.24268861 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Adsorption Consortium K. pneumonia P. bifermentans Sewage sludge Bran-based filler bacteria Salt-and-starch filler bacteria Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title | Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title_full | Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title_fullStr | Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title_full_unstemmed | Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title_short | Comparative screening study on the adsorption of Aqueous Pb(II) using different metabolically inhibited bacterial cultures from industry |
| title_sort | comparative screening study on the adsorption of aqueous pb ii using different metabolically inhibited bacterial cultures from industry |
| topic | UCTD Adsorption Consortium K. pneumonia P. bifermentans Sewage sludge Bran-based filler bacteria Salt-and-starch filler bacteria |
| url | http://hdl.handle.net/2263/92775 |