Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles
Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2023.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2023
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613613413367808 |
|---|---|
| access_status_str | Open Access |
| author2 | Lall, Namrita |
| author_browse | Lall, Namrita |
| author_facet | Lall, Namrita |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/93294 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:55.836Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/93294 Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles Lall, Namrita u17023409@tuks.co.za Reid, Anna-Mari Mandiwana, Vusani Diedericks, Bianca UCTD Medicinal plant science Tuberculosis Nanoparticles PLGA nanoparticles Antimycobacterial Cytotoxicity 7-Methyljuglone Poly-(lactide-co-glycolide) Sustainable development goals (SDGs) SDG-01: No poverty Natural and agricultural sciences theses SDG-01 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 Dissertation (MSc (Medicinal Plant Science))--University of Pretoria, 2023. Mycobacterium tuberculosis (M. tuberculosis) is a bacterium that causes the highly infectious disease, Tuberculosis (TB). During the 19th and early 20th centuries TB was known as the “white plague” due to the characteristic pallor of the patients infected with the disease. Tuberculosis was a major public health problem causing widespread illness and death. In the mid-20th century, effective treatments for TB were developed which included antibiotics and the Bacille Calmette-Guerin (BCG) vaccine. This led to a sharp decline in TB cases in developed countries such as Australia, Canada, European countries, New Zealand and the United States of America. However, even with the discovery and development of treatments for TB, it remains a main health obstruction in several parts of the world, especially in developing countries with limited access to healthcare. There are numerous reasons why TB as a disease is still problematic in South Africa. These include poor hygiene, overcrowding, poverty, and human immunodeficiency virus (HIV) co-infection. These factors make it difficult for people to access healthcare services and increase the risk of the spread of TB. Additionally, drug-resistant strains of TB have emerged in recent years, which require more complex and expensive treatment regimens. Medicinal plants have the potential to contribute to the management of drug-resistant strains of TB, it has been utilized for decades and has a major role in traditional usage in the treatment of diseases such as TB. Euclea natalensis A. DC (E. natalensis), is one such medicinal plant that is indigenous to South Africa. 7-Metyhyljuglone (7-MJ), a pure compound isolated from E. natalensis, has been shown to have promising antimycobacterial activity. The pure compound showed minimum inhibitory concentration (MIC) values against Mycobacterium smegmatis (M. smegmatis), drug susceptible M. tuberculosis (H37Rv) and multidrug-resistant M. tuberculosis (MDR11) of 1.6 µg/mL, 0.4 µg/mL and 1.6 µg/mL, respectively. 7-Metyhyljuglone has however also been shown to be highly cytotoxic as it exhibited a 50% inhibitory concentration (IC50) of 3.25 µg/mL on differentiated macrophage-like human histiocytic lymphoma (U937) cells. Literature indicated that using poly-(lactide-co-glycolide) (PLGA) nanoparticle formulations, as a delivery system, have shown the potential to reduce the toxicity of known toxic compounds. Therefore, this study aimed to determine whether PLGA nanoparticles containing 7-MJ, may have effective antimycobacterial properties, whilst being less cytotoxic than 7-MJ-only. This study found that the 7-MJ PLGA nanoparticles showed some antimycobacterial activity as they exhibited an MIC value of 125 µg/mL against M. smegmatis and MIC values of 250 µg/mL against both strains of M. tuberculosis (H37Rv and MDR11). The 7-MJ PLGA nanoparticles also showed to decrease the cytotoxic effect of 7-MJ on differentiated macrophage-like U937 cells with an IC50 of 247.17 µg/mL. Furthermore, this study is the first to report that the synthesized 7-MJ PLGA nanoparticles were successfully taken up within differentiated macrophage-like U937 cells, the cells which are most infected by M. tuberculosis. National Research Foundation [grant number: 98334] Plant Science MSc (Medicinal Plant Science) Restricted Faculty of Natural and Agricultural Sciences 2023-11-14T05:34:42Z 2023-11-14T05:34:42Z 2024-05 2023 Dissertation Diedericks, B 2023, Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles, MSc dissertation, University of Pretoria, Pretoria. A2024 http://hdl.handle.net/2263/93294 DOI: https://doi.org/10.25403/UPresearchdata.24551122.v1 * en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Medicinal plant science Tuberculosis Nanoparticles PLGA nanoparticles Antimycobacterial Cytotoxicity 7-Methyljuglone Poly-(lactide-co-glycolide) Sustainable development goals (SDGs) SDG-01: No poverty Natural and agricultural sciences theses SDG-01 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title | Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title_full | Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title_fullStr | Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title_full_unstemmed | Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title_short | Antitubercular activity of 7-methyljuglone-loaded poly-(lactide-co-glycolide) nanoparticles |
| title_sort | antitubercular activity of 7 methyljuglone loaded poly lactide co glycolide nanoparticles |
| topic | UCTD Medicinal plant science Tuberculosis Nanoparticles PLGA nanoparticles Antimycobacterial Cytotoxicity 7-Methyljuglone Poly-(lactide-co-glycolide) Sustainable development goals (SDGs) SDG-01: No poverty Natural and agricultural sciences theses SDG-01 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 |
| url | http://hdl.handle.net/2263/93294 |