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Quantitative detection of Theileria haneyi in South African horses
Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2023.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613629224845312 |
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| access_status_str | Open Access |
| author2 | Bhoora, Raksha V. |
| author_browse | Bhoora, Raksha V. |
| author_facet | Bhoora, Raksha V. |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94276 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:11.002Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94276 Quantitative detection of Theileria haneyi in South African horses Bhoora, Raksha V. u21700339@tuks.co.za Nicola, Collins E. Mbaba, Tshenolo Vincentia UCTD Theileria haneyi Equine piroplasmosis Field samples Quantitative real-time PCR South Africa SDG-03: Good health and well-being Veterinary science theses SDG-03 Dissertation (MSc (Veterinary Tropical Diseases))--University of Pretoria, 2023. Theileria haneyi is an apicomplexan parasite that is closely related to Theileria equi, a known causative agent of equine piroplasmosis. Theileria equi genotypes A, B, C, and D have been reported to occur in South African equids. Preliminary studies in South Africa indicated an association between T. equi genotype C and T. haneyi infections. The molecular distinction between these parasites is reliant on a nested PCR assay, which has been reported to be unreliable. A recently reported indirect ELISA based on the equi merozoite antigen (ThEMA-11) of T. haneyi can detect geographically diverse T. haneyi strains. Based on the exclusivity of the ema-11 gene to T. haneyi, we developed a TaqMan minor groove binder (MGB™) quantitative real-time PCR (qPCR) assay to amplify and detect the ema-11 gene. Published Thema-11 gene sequences were used to design primers for the amplification of the ema-11 gene from South African samples. Thema-11 amplicons were cloned and sequenced. An alignment of the South African ema-11 gene sequences with published sequences enabled the identification of a conserved region for the design of the qPCR assay. The T. haneyi ema-11 (Thema-11) qPCR assay was shown to be rapid, specific, and sensitive in detecting T. haneyi infections. The diagnostic utility of the Thema-11-specific qPCR assay was evaluated together with a T. equi ema-1-specific qPCR assay. Theileria haneyi was detected in 75% of the South African field samples screened, while the occurrence of T. equi based on the quantitative amplification of the ema-1 gene was much higher (100%). These results suggest that used in combination, the Thema-11-specific qPCR assay, and the T. equi ema-1-specific qPCR assay could detect and differentiate between T. haneyi and T. equi infections. National Research Foundation of South Africa (Grant number: 129240) Agricultural Sector Education Training Authority, and the Belgian Directorate-General for Development Cooperation through its Framework Agreement with the Institute for Tropical Medicine (FA4 DGD-ITM 2017-2021) Veterinary Tropical Diseases MSc (Veterinary Tropical Diseases) Unrestricted Faculty of Veterinary Science SDG-03: Good health and well-being 2024-02-05T08:04:14Z 2024-02-05T08:04:14Z 2024-04 2023-12-01 Dissertation * A2024 http://hdl.handle.net/2263/94276 10.25403/UPresearchdata.25039124 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Theileria haneyi Equine piroplasmosis Field samples Quantitative real-time PCR South Africa SDG-03: Good health and well-being Veterinary science theses SDG-03 Quantitative detection of Theileria haneyi in South African horses |
| title | Quantitative detection of Theileria haneyi in South African horses |
| title_full | Quantitative detection of Theileria haneyi in South African horses |
| title_fullStr | Quantitative detection of Theileria haneyi in South African horses |
| title_full_unstemmed | Quantitative detection of Theileria haneyi in South African horses |
| title_short | Quantitative detection of Theileria haneyi in South African horses |
| title_sort | quantitative detection of theileria haneyi in south african horses |
| topic | UCTD Theileria haneyi Equine piroplasmosis Field samples Quantitative real-time PCR South Africa SDG-03: Good health and well-being Veterinary science theses SDG-03 |
| url | http://hdl.handle.net/2263/94276 |