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Bovine and tick responses towards vaccination with Bm86 and its putative binding partners
Thesis (PhD (Genetics))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613519392800768 |
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| access_status_str | Open Access |
| author2 | Maritz-Olivier, Christine |
| author_browse | Maritz-Olivier, Christine |
| author_facet | Maritz-Olivier, Christine |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Genetics))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94454 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:26.341Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94454 Bovine and tick responses towards vaccination with Bm86 and its putative binding partners Maritz-Olivier, Christine marietteferreira2@gmail.com Stutzer, Christiaan Crafford, Jan Ernst Ferreira, Mariëtte UCTD Bm86 Multicomponent vaccine Rhipicephalus microplus DNA microarray Protein-protein interaction Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 Thesis (PhD (Genetics))--University of Pretoria, 2024. Rhipicephalus microplus and its associated diseases affect ~80% of the world’s cattle population, with a staggering financial impact, especially in developing countries. Despite the vast amount of research dedicated to tick vaccine development, there is only one commercial success, namely the Bm86-based vaccines. The efficacy of Bm86-based vaccines varies across different geographical areas, encouraging the development of improved vaccines. In this study we followed a novel and systematic approach to improve the efficacy of Bm86, the only antigen used in commercialised vaccine formulations against R. microplus. By means of a yeast two-hybrid approach we validated several protein-protein interactions of Bm86 and BmATAQ, respectively, previously identified by our research group. To improve the efficacy of the current Bm86-based vaccine we formulated a combinatorial vaccine in an attempt to target a complex of proteins possibly involved in the same biological pathway. Bm86 and its putative interacting proteins were evaluated in different combinations with some yielding significant results. The transcriptomic response of midgut tissue from R. microplus that fed on Bos taurus cattle vaccinated with Bm86 and its putative binding protein, KUBP was assessed. This allowed for the first time to identify differentially regulated transcripts, giving substantial insight into the mechanism by which the tick counteracts current stress from the immune response elicited by cattle upon vaccination. This research entailed a novel systematic approach that used a combination of in vivo vaccine cattle trials together with functional genomic techniques, permitting the prediction of putative antigens that would enhance vaccine formulation. To date, there is no vaccine available against ticks in South Africa. The development of a vaccine would lessen the pressure on ticks to evolve resistance to acaricides and would also lessen environmental contamination and economic losses brought on by R. microplus. National Research Foundation of South Africa Technology and Innovation Agency of South Africa Meat Industry Trust Genetics PhD (Genetics) Unrestricted Faculty of Natural and Agricultural Sciences 2024-02-12T08:02:57Z 2024-02-12T08:02:57Z 2024-04 2024-02-07 Thesis * A2024 http://hdl.handle.net/2263/94454 https://doi.org/10.25403/UPresearchdata.25127621 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Bm86 Multicomponent vaccine Rhipicephalus microplus DNA microarray Protein-protein interaction Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title | Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title_full | Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title_fullStr | Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title_full_unstemmed | Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title_short | Bovine and tick responses towards vaccination with Bm86 and its putative binding partners |
| title_sort | bovine and tick responses towards vaccination with bm86 and its putative binding partners |
| topic | UCTD Bm86 Multicomponent vaccine Rhipicephalus microplus DNA microarray Protein-protein interaction Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 |
| url | http://hdl.handle.net/2263/94454 https://doi.org/10.25403/UPresearchdata.25127621 |