Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa
Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Published: |
University of Pretoria
2024
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613500164014080 |
|---|---|
| access_status_str | Open Access |
| author2 | Ehlers, M.M. (Marthie Magdaleen) |
| author_browse | Ehlers, M.M. (Marthie Magdaleen) |
| author_facet | Ehlers, M.M. (Marthie Magdaleen) |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94537 |
| institution | University of Pretoria (South Africa) |
| last_indexed | 2026-06-10T12:37:08.061Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94537 Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa Ehlers, M.M. (Marthie Magdaleen) awsilen@yahoo.co.za Strydom, Kathy-Anne Ndlovu, Neliswa UCTD Staphylococcus aureus Cystic fibrosis Antibiotic resistance Virulence Genetic diversity Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2024. Introduction. Cystic fibrosis is a hereditary disease that results in ineffective mucocilliary clearance of secretions, creating an environment that allows Staphylococcus aureus to thrive. The aim of this study was to determine the phenotypic and genotypic characteristics of S. aureus isolates obtained from cystic fibrosis patients attending private clinics in South Africa. Materials and Methods. A total of 87 S. aureus isolates were collected. Phenotypic susceptibility testing was performed using the Vitek®2 automated system (bioMérieux, France) according to EUCAST guidelines. Multiplex-PCR assays were used to target antibiotic resistance genes and a selection of biofilm formation, haemolysins and cytoxin genes. Pulsed-field gel electrophoresis was conducted and a dendrogram constructed to assess the genetic relatedness of isolates. Whole genome sequencing was performed on four isolates to determine sequence types, the resistome and virulome of the isolates. Results. Phenotypic antibiotic susceptibility testing showed the highest resistance against erythromycin in 62.1% (54/87) of isolates. Multiplex-PCR assay results showed 11.4% (10/87) of the S. aureus isolates were MRSA; and the most prevalent macrolide and tetracycline resistance genes was ermC 36.8% (32/87) and tetK 2.3% (2/87). The biocide resistance gene, qacC was detected in 4.5% (4/87) of the isolates. The most prevalent virulence gene was: hla 48.2% (42/87). Sequence types detected were pandemic strains ST5 and ST36, and livestock associated strain ST398 as well as a novel ST. The S. aureus isolates from CF patients in this study were highly diverse, indicating limited spread from clinical settings. Multidrug resistant isolates were detected which limits treatment options for these patients; the isolates also harbour virulence genes that can increase the severity of the disease and potentially increase the morbidity and mortality among these patients. National Health Laboratory Service (NHLS) The University of Pretoria School of Medicine Research Committee Medical Microbiology MSc (Medical Microbiology) Unrestricted Faculty of Health Sciences SDG-03:Good heatlh and well-being 2024-02-13T09:48:07Z 2024-02-13T09:48:07Z 2024-04 2024 Dissertation * A2024 http://hdl.handle.net/2263/94537 10.25403/UPresearchdata.25211204 © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Staphylococcus aureus Cystic fibrosis Antibiotic resistance Virulence Genetic diversity Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title | Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title_full | Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title_fullStr | Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title_full_unstemmed | Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title_short | Phenotypic and genotypic characterisation of clinically relevant Staphylococcus aureus isolates from cystic fibrosis patients from private clinics in South Africa |
| title_sort | phenotypic and genotypic characterisation of clinically relevant staphylococcus aureus isolates from cystic fibrosis patients from private clinics in south africa |
| topic | UCTD Staphylococcus aureus Cystic fibrosis Antibiotic resistance Virulence Genetic diversity |
| url | http://hdl.handle.net/2263/94537 |