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Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984
Dissertation (MSc (Biochemistry))--University of Pretoria, 2024.
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| Format: | Thesis |
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University of Pretoria
2024
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| _version_ | 1867613662419615744 |
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| access_status_str | Open Access |
| author2 | Gaspar, A.R.M. (Anabella Regina Marques) |
| author_browse | Gaspar, A.R.M. (Anabella Regina Marques) |
| author_facet | Gaspar, A.R.M. (Anabella Regina Marques) |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biochemistry))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94669 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:42.666Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94669 Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 Gaspar, A.R.M. (Anabella Regina Marques) lizzyvh1@gmail.com Bester, Megan J. van Heerden, Lize Janeska UCTD Antibacterial Staphylococcus epidermidis Antimicrobial peptide Molecular dynamic simulation Vancomycin Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 Dissertation (MSc (Biochemistry))--University of Pretoria, 2024. Antimicriobial resistant Staphylococcus epidermidis infections are rapidly increasing. A promising research avenue is the use of antimicrobial peptides (AMPs). From AMP OsDef2, C-terminal fragments Os and Os-C were generated, but activity loss in physiological salt environments limits clinical applications. As an attempt to increase the antimicrobial activity under these conditions, Os-C was tagged on the N-terminal with five tryptophan residues. The minimum inhibitory concentrations (MICs) for the anti-planktonic activity of W5(Os-C) (W), vancomycin (V), and combination ratios 3V:1W and 2V:2W were 2.44 µM, 3.83 µM, 3.31 µM and 3.63 µM respectively. The biofilm inhibition concentrations (BICs) of W5(Os-C), vancomycin, and combination ratios 3V:1W and 2V:2W were 3.81 µM, 3.06 µM, 3.48 µM and 4.51 µM respectively for cell viability, and 3.76 µM, 3.07 µM, 6.68 µM and 4.40 µM respectively for biomass inhibition. Antimicrobial combinations failed to achieve synergism. In silico studies revealed that W5(Os-C) formed dimers or trimers and inserted into a model Gram-positive membrane regardless of salt concentration. Peptide-peptide interactions in a physiological salt environment were both hydrophilic and hydrophobic, but mainly hydrophobic in a higher salt environment. Irrespective of salt concentration, W5(Os-C) inserted into the Gram-positive model membrane via the N-terminal tryptophan tag but inserted deeper in the higher salt environment. In conclusion, N-terminal tagging of Os-C with tryptophan residues increases the anti-planktonic and biofilm inhibitory activity, mediated by dimer/trimer formation and N-terminal insertion into S. epidermidis model membranes. University of Pretoria National Research Foundation Biochemistry MSc (Biochemistry) Unrestricted Faculty of Natural and Agricultural Sciences 2024-02-16T07:36:32Z 2024-02-16T07:36:32Z 2024-04-16 2024-02-15 Dissertation * A2024 http://hdl.handle.net/2263/94669 https://doi.org/10.25403/UPresearchdata.25225163 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Antibacterial Staphylococcus epidermidis Antimicrobial peptide Molecular dynamic simulation Vancomycin Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title | Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title_full | Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title_fullStr | Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title_full_unstemmed | Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title_short | Antibacterial activity of synthetic peptide W5(Os-C) against Staphylococcus epidermidis ATCC 35984 |
| title_sort | antibacterial activity of synthetic peptide w5 os c against staphylococcus epidermidis atcc 35984 |
| topic | UCTD Antibacterial Staphylococcus epidermidis Antimicrobial peptide Molecular dynamic simulation Vancomycin Sustainable Development Goals (SDGs) SDG-03: Good health and well-being Natural and agricultural sciences theses SDG-03 SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 |
| url | http://hdl.handle.net/2263/94669 https://doi.org/10.25403/UPresearchdata.25225163 |